10 20
30 40 50
60 70 80
90
100
110
120
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
Cryptosporidium
parvum MSVLKGALAVLMKQEVLTLGEYLEQKRKIRVFKEEGLYNDSSGELRRLLEETLILEDPSIPKALKEGFLGLRELTFNSLDNSLDINGDRRKLLGINIEKLLEWSSILGQNYFSSPRNQSS
Cryptosporidium
hominis MSVLKGALAVLMKQEVLTLGEYLEQKRKIRVFKEEGLYNDSLGELRRLLEKTLILEDPFMPKALKEGFLGLRELTFNSLDNSLDINGDRRKLFGINIEKLLEWSSILGQNYFSSPRNQSS
Giardia
lamblia ------------------------------------------------------------------------------------------------------------------------
Giardia
intestinalis ------------------------------------------------------------------------------------------------------------------------
Leishmania
amazonensis -----------------------------------------------------------------------------------------MSASFPSIPGFAGPLSLKAFLEEYFGLHLTF
Leishmania
infantum -----------------------------------------------------------------------------------------MSASFPSIPGFAGPLSLKAFLEEYFGLHLTF
Trypanosoma
brucei -----------------------------------------------------------------------------------------MAQMFPSVPGYDGPFSLKDFLHNYIGLQLRW
130
140
150
160
170
180
190
200
210
220
230
240
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
|-------------------------------------------------------------------------------------------------Mo
Cryptosporidium
parvum MSSGKRSRNTEYNETLSPSFITD-QIKNIIPDSEKDS-----------------------------------------------------LTYQVQDFELLLRK--HFPRIRIFHS-GSY
Cryptosporidium
hominis MSSGKRSRNNEYNETLPPSFITD-QIKNIIPDSEKDS-----------------------------------------------------PTYQVQDFELLLRK--HFPRIRIFHS-GSY
Giardia
lamblia --------------------MDFPLSLLDANVSKAIF-----------------------------------------------------ILLQGEPEQASLWS--LILQLRTPGQ-GSS
Giardia
intestinalis --------------------MDFPLSLLDANVSKAIF-----------------------------------------------------ILLQGEPEQASLWS--LILQLRTPGQ-GSS
Leishmania
amazonensis AAETASPSPRAAATAETPSAAG-FRALRDVVLPPNQSFLVVVYVALHASSSPPPTTAHASPTPATPDPGCAAPPTGLGRLRQPLAHQTAVSTAHDTCVTRCNPSDRQNPFCLSSSSTGKN
Leishmania
infantum AVETASPSPRAAAAAEAPSAAG-FRALRDVVLPPNQSFLVVVYVALHASSSPPPTTAHASPTPPTPAPGCAASATGFGRLRQPLTHQTAASTAHDTYVARCSPADRPNSSCSSSSSTGKN
Trypanosoma
brucei STPQENSTTLPPSNSN-----------VVVVPPTGNFHVVVYVSRSAPPP----------------------------------QRSVRSVAVLSSC----------------------S
250
260
270
280
290
300
310
320
330
340
350
360
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
tif GQ--------------------------------------------------------------------------------------------|
Cryptosporidium
parvum SGLRTSFDTIKPKKYSFCN---STLYKFQ-NLHAWNT-----LLSKI-GPIEILFLFVCCIIFRILGDHSE-ILIQQAGRMLTNDFLEELARLYETGPK--KTNSFVSSSSLTPILTIEE
Cryptosporidium
hominis SGLRTSFDTIKHKKYSFCN---STSYKFQ-NLHAWNT-----LLSKI-GPIEILFLFVCCIIFRILGDHSE-ILIQQAGRMLTNDFLEELARLYETGPK--KTNSFVSSSSLTPILTIEE
Giardia
lamblia FASCA-----------------SLIYKDLKGLSSWIT-----EAAFI-PFLRRLGFCSLYLSYG---HDVL-LELFKYPIVLTSTVSGKRVLLSEPQMP---------------------
Giardia
intestinalis FASCA-----------------SLIYKDLKGLSSWIT-----EAAFI-PFLRRLGFCSLYLSYG---HDVL-LELFKYPIVLTSTVSGKRVLLSEPQMP---------------------
Leishmania
amazonensis GNARSPWCASASWLLYTNTSHRPFTDALLRHP--WRASFSACLGPAAMGFIE-MY----CPIVLQLEAMAGGVQVIGPALKHVALETSFSATAQLSEMKGLPPRGSASVSSRGVKRAVDT
Leishmania
infantum GNARGLWRSSAFWLLYTNTSHRPLTDALLRHP--WWASFAACLGPAAMGFIE-MY----CPIVLQLEAMAGGVQVLGPALKHAALETSFSATAPSSEMKGPPPQGSGYVSSRGVKRAVDT
Trypanosoma
brucei NKMDG---DHPQPSVPTHFHGRPLTSSVLKHP--FWDALFSQIGPTAVSFIV-LW----APIVVQFEASGGGLQVLGPPLKR-----------------SASCAVKRQPGAG--------
370
380
390
400
410
420
430
440
450
460
470
480
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
Cryptosporidium
parvum KEEVKPLDETPRQVNKRSGGIIKDSRLSKIYKIDIPYRSTFLYCDHFSKRGGLPCLSVLRLLPPNLLGARTLLRFIIQTDHIFKDHNRQSLIGLLGSYEMTKFSRVKCKLASAMMEEFQN
Cryptosporidium
hominis KEEVKPLDETPRQVNKRSGGIIKDSRLSKIYKIDIPYRSTFLYCDHFSKRGGLPCLSVLRLLPPNLLGARTLLRFIIQTDHIFKDHNRQSLIGLLGSYEMTKFSRVKCKLASAMMEEFQN
Giardia
lamblia ---------------------------------------------------------------LAFPPCLHDKSVSSMVRASTVQYASNCLEEFISMIQEHPMNVRFIFFCKTRPYIRAF
Giardia
intestinalis ---------------------------------------------------------------LAFPPCLHDKSVSSMVRASTVQYASNCLEEFISMIQEHPMKLRFIFFCKTRPYIRAF
Leishmania
amazonensis GE-CSAPLPLQKQRRVEAVASPPKAGRRLQREVAPSHRPCRDDSFSSPVNRAAMPATWAAALTRTDDPRTRLYSVRVSDSDCTGDG-GGALPLPAGSLEAHWLPRHPRSLHRVLQAALPK
Leishmania
infantum GE-CSGFLPLQKQRRVEAPP-KAKAGRRLQRGVVPCSRPRRDDPFASPVNRAAMPVTWAAALTRTDVPRTRLYSVRVSDSDGAGDGSGGALPLPAGFLEAQWLRRHPHSLHRVLQAALPK
Trypanosoma
brucei ---WWEGKLKVSRTETLTQND---------------------------PHCFCIEGTSSPVALYRLNVPRLHLVEQSPLRST------ADARHAVTLILEQLWIEHHKSSCSPSSDRDIS
490
500
510
520
530
540
550
560
570
580
590
600
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
|--------------Motif CP---------- -----
Cryptosporidium
parvum L--------------------------------------------------------------------------------LLNI------------RN-TSP-VN-FLD---QI-CPIE
Cryptosporidium
hominis L--------------------------------------------------------------------------------LLNI------------RN-TSP-VD-FLG---QI-CPIE
Giardia
lamblia HSLALLGSATAIWHLSLLPSQCLTAFYQANPLVTFKFPYNAFSTRSFSSLLQLCTSRGRLMPNNAINSNAKARITRLISIFRSKM------------KR-LSHNLT-FL----KD-KKPQ
Giardia
intestinalis HSLALLGSATAIWHLSLLPSQCLTAFYQANPLVTFKFPYNAFSTRSFSSLLQLCTSRGRLMPNNAINSNAKARITRLISIFRSKM------------KR-LSHNLT-FL----KD-KKPQ
Leishmania
amazonensis RAAYGASTRHYCAGTGERGTGCTSVKNISMWHVAHVFRWLV----------------------------------------LQPSQSGEVAFQTTSPKFDLPSYLRRFLSTDVDQ-CSRL
Leishmania
infantum RVAYGASARRYSVGASERVTGCTSVEDIPMWHVAHVFRWLV----------------------------------------LQPPQSGADASPTTPPKFDLPSYLRRLLSTVVDQ-CSRL
Trypanosoma
brucei QRLCG------VATGSATNCCGDSCVVLAQARNQVNAV-------------------------------------------FPYTL-LPVHDDAVSGNGTVKEYLIHVLHCALGSVC-RM
610
620
630
640
650
660
670
680
690
700
710
720
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
| |--------------------------Motif QFP-------------------------------
Cryptosporidium
parvum PIKDSDLQNFNKL----------------------------------------PIPCFETSSTRVVNF-------LRLYLIKVLPKNILGTFKNFKTFINKKIPIIVNLHIRETFKIQHA
Cryptosporidium
hominis PIRDSDLQNFNKL----------------------------------------PIPCFETSSTRVVNF-------LRLYLIKVLPKNILGTFKNFKTFINKKIPIIVNLHIRETFKIQHA
Giardia
lamblia SLLQSFGFDTHFRVFLDE-----------TGTL--------------------RHAPLPLSVHKITLY-------LMLYCKSISAFSILG-KRNARTFH-SHLFRFISQPMNGFMTLQEL
Giardia
intestinalis SLLQSFGFDTHFRVFLDE-----------TGTL--------------------RHAPLPLSVHKITLY-------LMLYCKSISAFSILG-KRNARTFH-SHLFRFISQPMNGFMTLQEL
Leishmania
amazonensis DLRGAALRHTGYLEEAFR--------RQQQGVEPWDVQRL---STPVDVVVS--YLRTLLPTLRWAPL-------KEANNGLFWGRDAAGSERVLDALM-RAVRGWLIAGRQAVVPVSRF
Leishmania
infantum DLRGAALKHTGYLEEAFR--------RQQQGVEPWDVQRL---STPVDVVVS--YLRTLLSTLRWAPL-------KEAKNRSFWGRDAAGSERVLDALM-GAVRNWLIAGRHAIVPVSRF
Trypanosoma
brucei NIRGAAIKHTEFLEKRGKSVTSISQKAQTAGQENKMRAIPVGELTVPEPVVAS-YLETLFNMMWWRVPPRFVGGAVTADRVKFWGEEGT----VLTRLR-EVTVDWLCCGRHEVFLLSHF
730
740
750
760
770
780
790
800
810
820
830
840
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
------------| |----------------------Motif T------------------------
Cryptosporidium
parvum MNGIEVSN--WVNRILEEKSYQFIKESKNLINSNQRSKKVPNNKTRSSIKKSLISLGKTYLARNIYFL-IVYLVFP-----ILRRHF----YATEIE-GSNKV-----RYFRHPVWIKIV
Cryptosporidium
hominis MNGIEVSN--WVNRILEEKSYQFIKESKNLINSNQRSKKVPNNKTRSSIKKSLISLGKTYLARNMYFL-IVYLVFP-----ILRRHF----YATEIE-GSNKV-----RYFRHPVWIKIV
Giardia
lamblia TSSISTKEF--L-LPATTVSKAEGEVRHHLLAYFVV------------------------------YL-MDFVILP-----ALYQSFSAVSMHTELSNRIETANSMVPALLSRSLWVSYK
Giardia
intestinalis TSSISTKEF--L-LPATTVSKAEGEVRHHLLAYFVV------------------------------YL-MDFVILP-----ALYQSFSAVSMHTELSNRIETANSMVPALLSRSLWVSYK
Leishmania
amazonensis LDGVPVAQVPWL-NGFYTTTPSLPFSDATSSASRHARRERSQVQQRV-------------------WLQFVLFLTQDILPFLLRASF----TITWSSKNTHKL-----LFFPAFVWRRLV
Leishmania
infantum LDGVPVAQVPWL-NGFYTTTPSLPFSAATSSAARHARRERSHVQQRV-------------------WLQFVLFLTQDILPFLLRASF----TITWSSKNTHKL-----LFFPAFVWRRLI
Trypanosoma
brucei LDGVPVSRIPWL-RGFYTKEP-----------ARKRRSMIQQRV----------------------FLQLVLFLYQCVVPFLIRRSF----HVACTSKSPYIF----FFIPRAVWVRLTR
850
860
870
880
890
900
910
920
930
940
950
960
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
--------| |--1-||---2--|
Cryptosporidium
parvum RQADKWYLESILKGIQSKDYIMNLSSIQEISKLIDKNSEFSENIPK-------------------------------------------------------IRWVPKSKGLRPLINLSKV
Cryptosporidium
hominis RQADKWYLESILKGIQSKDYIMNLSSIQEISKLIDKNSEFSENIPK-------------------------------------------------------IRWIPKSKGLRPLINLSKV
Giardia
lamblia NYLIKSSARLEIAATYEFVCSLSNLHSIPLTNEDAVNRIFWKQVSPGR-----------------------------------------------------LSFVYKSNGRVRPLCNFSF
Giardia
intestinalis NYLIKSSARLEIAATYEFVCSLSNLHSIPLTNEDAVNRIFWKQVSPGR-----------------------------------------------------LSFVYKSNGRVRPLCNFSF
Leishmania
amazonensis RREVRRTRSCHAPRSQMSLAEERTRMSGADHAALVSAPNACGGAVAAAFPSPHSAASNASAAISAPRDEWRAVRTGGALAHWSARATLATRGGGASCLYAGVRFRPDRRKLRPIAVVRSA
Leishmania
infantum RREVRRTRSCGAPRSQMPLAGESARSVGAERVALSSAPCACEGAATAALPTPHSAASKASAAISAPRDVWRAVRTDGVLAHRGARATLATRGGGASCLYASVRFRPDRRKLRPIAVVRSA
Trypanosoma
brucei REMRRVCIRRVKRMRSDTLSDPQMRGHAGLPPMALERVTSEGLMQLIGPSSQPVGDDS----------GGVSDVCKLRDSSSPLLYSD-------------VRFLVDGSKLRPIARPRFG
970
980
990
1000
1010
1020
1030
1040
1050
1060
1070
1080
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
Cryptosporidium
parvum GSGQILQQNLERDHTCEEKKKIGFCDCNSVWTGGDLPTSWINNNYYYNYFNSNGNNGIVGNNHNYGHINNIGGKNFTSFSSQNCQIMTPPGRLGVGLGFGNYNIGIGINSGSSVPTTPIN
Cryptosporidium
hominis GSGQILQQNLERDHTCEEKKKIGFCDCNSVWTGGDLPTSWINNNYYYNYYNSNGNNGTVGNNHNYGHINNIGGKNFTSFSSQNCQIMTPPGRLGVGLGFGNYNIGIGINSGSSVPTTPIN
Giardia
lamblia CSRRHRVS----LNSFLRSALQVIIFELERP-------------RNRYLQAHLAKNLGHIAVDY-----------AAWLDATY------TENPE--------------------------
Giardia
intestinalis CSRRHRVS----LNSFLRSALQVIIFELERP-------------RNRYLQAHLAKNLGHIAVDY-----------AAWLDATY------TENPE--------------------------
Leishmania
amazonensis SLRSLKEMARGSPSPYSHASAIVRLLRRLGCSDADGQLPAATATLLRQVQARSRHNRRTGVHRSAHPLPPHLPHKAALQDALRCLVSGVEEQRVRDGLPRLSNLSHQDEYAELRSFCEEV
Leishmania
infantum SLRSLKEMARGSPSPYSHASAIVRLLCRLGCSDTDGQLPAATATLLRRVQARSRHNHHTGVHRFPHPLPPHLPHKAALRDALRCLVSGVEEQRVRDGLPRLSNLSHQDEYAELRSFCEEV
Trypanosoma
brucei WQRSLLKAADG---------------------------VGSSTLASALAKAGKYLATDGKAMWPS-------PNSAVLRDALRCLDAGVEER----------RITTGARQRAIGSHNDEY
1090
1100
1110
1120
1130
1140
1150
1160
1170
1180
1190
1200
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
|--------------Motif A-------------
Cryptosporidium parvum KVMRRPSSNRIIDIRRPSTNNMLFYPSKILRSFLLRMTGKNHLGA