10 20 30 40 50 60 70 80 90 100 110 120

....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|

structure

Kluyveromyces lactis ------------------------------------------------------------------------------------------------------------------------

Kluyveromyces wickerhamii ------------------------------------------------------------------------------------------------------------------------

Kluyveromyces nonfermentans ------------------------------------------------------------------------------------------------------------------------

Kluyveromyces marxianus AAUUGUAUACUUUUUAAACAACCUAAACAUGUUGUUACGUUCUAUUAUGUUCAGUAACAAUAUAGACAAUUUACCCUGCGUGUUCGAUUCUAUUUGCCAAAGCCUAGAGAGUGAUUGAGG

Kluyveromyces dobzhanskii ------------------------------------------------------------------------------------------------------------------------

Kluyveromyces aestuarii ------------------------------------------------------------------------------------------------------------------------

130 140 150 160 170 180 190 200 210 220 230 240

....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|

structure

Kluyveromyces lactis --GAAUAUAUGAUGGGUGCGGAAUCACGAUCGGUAUUUGUGUGAUGCAAUUUCUGGGGUGGUAAGGACCAGUGCCACACUUGGGUUUGAUUUUCCUCUCAUCGAGUUUCGUGUGCGCAUG

Kluyveromyces wickerhamii --AAAAUUUACUGCGAUCUCAACGAACAUAAGCUACUAAAUCUAUUUCAAUUAUUCCAAAACUUAUUUCACGAUGGGAACAAGGAGGCCUUUGGUAGAUCUGGUUGCUUACGAGGAGGUA

Kluyveromyces nonfermentans ---------------------------------------------------------------GUAAAGAAGAUGUGUUAGGUUUACAACGCUCUUCUCAGCAUUGUAUUGAAGUUUGUU

Kluyveromyces marxianus UGGUUAGGACCAGUACCACGUAUGAGACGUGUUUAACUUUUUCCAAAUGCUGACAAGGUAUAUGUCUCGUAUUUCCCUGACGUUCUCUUUACGCCUGGGAAUUGACUGAGUGGACACUC-

Kluyveromyces dobzhanskii -GAACUCCAUGAGUUCGUAAUCAGCAAGGAUUGUGGUAUUAUUUGCUGAAAGACGAAGUGUGAACGAAGUUUGAGGUGGUAAGGACCAGUGCCAUGCUUGAGUGUUUCUUUCUGAGUUUU

Kluyveromyces aestuarii -------------------------------------------------------AGCAAAGCAUUUGAAAGCUGAUUUGAAUUUCUAUCGUUCUUUUCAAAACUUCAUUGAAGAUAGUU

250 260 270 280 290 300 310 320 330 340 350 360

....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|

structure

Kluyveromyces lactis UUCGUCUCUCACAUCGAGUUAUACUCUCAUCUUCUAUACAUUAGAAGGAUGCAUCAGCAGUUUGAUCUGCUGAAAGAGUUCAUACACCCAAUUAUUGUUUAAAGGCCUGGUGUUGGGUAG

Kluyveromyces wickerhamii AUGGAGCCAGUACCUUAUCGCCACUUUUCGAUUGACACCAAAGCUCCCACAACGCGAACGUGAAACGCAUGACUGUUCU-----------------------------------------

Kluyveromyces nonfermentans UAGAUAGAGGCGGUUUUGGAACCUCACCUGAUGUACUCUUUCAAAUAUUC-UGUGAUAUU-----ACAAUGUUGCCCUUAGUGAUUG---CAUGUGACCUGUUUUGCGGGAGAAGCGGAC

Kluyveromyces marxianus CAAAGCUUUAGAUAUACCUGGC--------------------------------------------------------------------AUGCUAGAUCGUAUGCAUAUAUUUACGGAU

Kluyveromyces dobzhanskii AGGUGUGCAUGUUUGUGUGUUUGACGACAUAGUCCCAUUCGUGUAUCUGACAUACCAGAAGUACACGGAAGGAAACUGAGUUUGUUGAAUGAAGUGAGGUGCAGCCACACAGCGAUCAAA

Kluyveromyces aestuarii UAAUUGGGGGCGGUUUUGAACCCUGGCCUGAUAGUUCCUUCAAAAGGUGCAUUUGAUUCUCACGUACAACUUCGCCUCACCCCAUUGAAGUUUGCCAGUAAUUCAGCUAAAUAAGCGGAC

370 380 390 400 410 420 430 440 450 460 470 480

....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|

structure

Kluyveromyces lactis UAUUUCUAACGGAUUUUAGAAAUGCUAUGACAACAAUACCUUUAGAAUUAUAUUGUGUGGAUAUUUACUUCUUGUUAAACAGACUGUGACAUAUCUCAAUGAGAGGGGAUUAUUCUCGUU

Kluyveromyces wickerhamii ---------------------AUGCACUGUUUAUUUACAAAGCAUUCGGAAAAUACGAUGUAAAUAUGAGGUGUACGCACAAAGUUGUCGAUUGUAAAUGACCUAUCUAACUCAGUUCUA

Kluyveromyces nonfermentans CUUACGACGCUGAAUAGGAAAAUUAGUUUGAACUAAUAUUAUAUCACAUGGAGUUUCAAGGGGUGGAUAAGGUAGCAGUCUAACUGCCUGUCAGGGUAUCAUAUGGAAGUGUAUAUCA-G

Kluyveromyces marxianus UAAAGAUAUGUGUAUGAAUAUGGCGCUAGGAAUUUUGUGCUUAAAAGAGAGUUUGCGUUUAA-UAGGUCUUUCGCAGACUAUACCGGAUAAUACCAAUUUCGGAGGCAUGAGGAUUGGGG

Kluyveromyces dobzhanskii CCAGUGCUGUUGUUCGGUGCUUCUGACGGAUUUGGAAAACCUGAAUUGAUACGUGCCUUUUGAAAUUUUACUGGGUUGCAGUGUACCUUACUUUACCAGACUGACUCUUGUCUCGAUGGG

Kluyveromyces aestuarii CUUACGAAACUGAACUCAGGCAAACUC-CCUGUAUGAAAAUGAC--UGCUUGGAGCUGUUUGGA-UGAAGAAUGUAAGGGAUAGAUCUUAUGCAAUUGCCUUG---AGAAGUGAACUUCA

490 500 510 520 530 540 550 560 570 580 590 600

....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|

Template

structure ||||||||||||||||||||||||||||||

Kluyveromyces lactis UUUGGAGUUAUUCUGAUGCCUGUUGCUCUUGCUCCUACGCAUAGAAGCAUAGCAUUGAACUCCAUGGUGUGGUACUUCAAAUCCGUACACCACAUACCUAAUCAAAACCUCCUC-GCAAC

Kluyveromyces wickerhamii UAGGCCUUACACAUGUUUCAAACCUUGGUCUAGCUUUAAUAUGGUGCAGUGCGAAGAGUUAACAGAUUUGGUACUAU-AAAUCCGUACACCACAUACCUAAUCAAAACCGCCUU-AGAAC

Kluyveromyces nonfermentans GUGUA-----------------------------------------------------------------------UCAAAUCCGUACACCACAUACCUAAUCAAUCCUUUUUUUAGGAC

Kluyveromyces marxianus AGGCUCGCGCUC-AGUUAGAGAUGGUUAAGC--UCUCAAUG-GGAGUUUA-CAUCCAUUUUAUAAACGUGGUACUUUCAAAUCCGUACACCACAUAACUAAUCAAAACCGCUUC-UAAAC

Kluyveromyces dobzhanskii GAAAUACUUUAGUAUUGAAGUUCUUUCAACGUUGCACUCUUGUGCACAAUGCCUGGAAUUGUAGCGUUGAUCUUCAUAGCAU-GGCACUUCAAAUCCGUACACCACAUACCUAAUCAAAA

Kluyveromyces aestuarii -GGUGUA---------------------------------------------------------------------UCAAAUCCGUACACCACAUACCUAAUCAAUCCUUUUU--GGAAC

610 620 630 640 650 660 670 680 690 700 710 720

....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|

|----CS2----|

structure ((((((((( (((

Kluyveromyces lactis GGCUUCCAUAUUCUCGUCUGUAUUCAAUCG-UGGAUAGUGGUUUCAGCUCGACGUGGGUAUGCUAGUGACUCAGAGUUUGGAUGUACUA-GGUUUCAUAAG-------------------

Kluyveromyces wickerhamii GGUU---AAACUCUGGUGUGUUUC-AUUUG--GCACUGUGGUACGGCCGUUA----GCUACGGCUCUAAUUCAGGGUUUAGA----UCAAGUUUACAAACGGAU----------------

Kluyveromyces nonfermentans AGCCGU--GUUUCUGUUUCAUUGACAACGA----AUAGCGGC-GGAUAAAGCCAGCACUAA--UCGGAAUUGAGGGUUUGGA----UCAAGGUUACAUCAG-------------------

Kluyveromyces marxianus GGCUUUAAA-UUCAUAUCUGUAUCAACUUGAGGGAUAGCGGCGUCGAUUCGA-AAGGGCAA--CAGUGAUUCAGGAUGUGGAAAAGUCAAGGUUGCAUAAG-------------------

Kluyveromyces dobzhanskii CCGCUUUGAAACGGCUCCCAUUCUCGUCUGUUUCUACCCUGGGAUAGUGUUUUCAAUUCGACGUGGGUAAGCUGCAGAUUCAGGGUUUGGGGCACUAGGUUGCAUAAGAUUUGCAGCAAC

Kluyveromyces aestuarii UGCCAU--AUUUUUUGUUCACUGGCAACCA----AAAGCGGCCAAAGAGGGCUAGCAUCAG--UUGGAAUUAAGGGUUUGGA----UCAAGGUUACACAAA-------------------

730 740 750 760 770 780 790 800 810 820 830 840

....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|

|------CS2------|

structure ))))))) )))))

Kluyveromyces lactis ---------------------------------------------GAUACCCAACUG-------------GAAAUGUUGC-GGAAAUAUCACAUUCGUCCUUGCUCCAUUGUUAACUCAG

Kluyveromyces wickerhamii --------------------------------------AACUUGUGUUCUGCAGGU---------------AACUGUGACCAAGUUGCAUCACUUAUGCUUGAUUCUAUGGUUAACCCAG

Kluyveromyces nonfermentans -----------------------------------------------------------------------GAAGGUUUU--GCUUUCAUAUUUUCUUUGCGAAUCCAUGGUUAACCCCG

Kluyveromyces marxianus --------------------------------------GAUGCAUGAGAUGAAAUUC--------------AAAUGUGUCAAAAACUCCUCGUUCCAUCGUUAAUCCGGUGU-CAUUUAG

Kluyveromyces dobzhanskii AUUCAGUUUCCAUGUAUGGUGCGAAAAGGCAGUAAUACUGUAGGAGAAGGCUGAUCCUUAAUUUUUUAAGUUCAGCUGGAAUGUUGCCAUAAAAAUCACCAUUAUCCUGGCCCCAUUGUU

Kluyveromyces aestuarii -----------------------------------------------------------------------GAACAUUAC--AUUUUCACAUUUGCCUUGCAUUUCCAUGGUUAACCCAG

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