10 20
30
40 50
60
70 80
90
100
110
120
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
|-NL--| |---TruB I---|
Homo sapiens
MADAEVII-LPKKHKKKKERK---SLPEEDVAEIQHAEEFLIKPESKVAKLDTSQWPLLLKNFDKLNVRTTHYTPLACGSNPLKREIGDYIRTGFINLDKPSNPSSHEVVAWIRRILRVE
Rattus norvegicus
MADAEAAMTFPKKHKKKKERK---PLPEADVAEIQHAEDFLIKPESKAAQLDTSQWPLLLKNFDRLNVRTTHYTPIPCGSNPLKREIGEYVRTGFINLDKPSNPSSHEVVAWIRRILRVE
Mus musculus
MADAEVI-TFPKKHKKKKDRK---PLQEDDVAEIQHAEEFLIKPESKVAQLDTSQWPLLLKNFDKLNVRTAHYTPLPCGSNPLKREIGDYIRTGFINLDKPSNPSSHEVVAWIRRILRVE
Bos taurus
MADAEVLI-LPKKHKKKKERK---SLPEEAVAEIQHAEEFLIKPESRVAQLDTSQWPLLLKNFDKLNVRTTHYTPLPCGSNPLKREIGDYIRTGFINLDKPSNPSSHEVVAWIRRILRVE
Gallus gallus
MADGDG---SSVKKRRKKDKR---SLPDEDVADIQHTEEFLIKPESRVAQLDTSQWPLLLKNFDKLNVLTTHYTPLPSGANPLKREISDYVRSGFINLDKPSNPSSHEVVAWIRRILRVE
Danio rerio
MADTES-----KKEKKRKSKK----ISDEEVGDIQESGDFLIKPESKVASLDTSQWPLLLKNFDKLNIRTAHYTPLPHGSNPLKRGIHDYVRSGFINLDKPANPSSHEVVAWIKRILRVE
Drosophila melanogaster MADVEV-----RKEKKKKKIKEEP-LDGDDIGTLQKQGNFQIKPSSKIAELDTSQWPLLLKNFDKLNIRSNHYTPLAHGSSPLNRDIKEYMKTGFINLDKPSNPSSHEVVAWIKKILKVE
Anopheles gambiae
MEVDI-PTSPIKKEKKKKKIKQEPDTEIENLGEIQKSGDFQLKPSSAIASLDTSKWPLLLKNFDRLNVRTNHYTPLPFGSSPLNRKINDYVSAGFINLDKPSNPSSHEVVAWIKRILKVN
Aedes aegypti
MSDYEMGSPTIKKEKKKKKIKQEPGAEIENLGEIQKSGNFQIQPSAAIAKLDTSQWPLLLKNFDRLNVRTNHYTPLPFGSSPLNRKIKDYVTSGFINLDKPSNPSSHEVVAWIKKILKVD
Culex pipiens quinquefasciatus MG-----SPHIKKEKKKKKIKQEPGVEVENLGEIQKSGDFQIKPSEAIAKLETSKWPLLLKNFDRLNVRTNHYTPLPFGSSPLNRKIKDYVTSGFINLDKPSNPSSHEVVAWIKKILKVE
Caenorhabditis elegans MG---------KKDKRSK-------LEGDDLAEAQQKGSFQLPSSNETAKLDASQWPLLLKNYDKLNVRTNHYTPHVEGVSPLKRDIKNYISSGFFNLDKPSNPSSHEVVSWIKRILRCE
Schizosaccharomyces pombe MTD-----------------------------THPGVDFMIKPEATSASKIDTAEWPLLLKNFDKLLVRTGHYTPIPCGNNPLKRPIAEYVSSGVINLDKPANPSSHEVVAWVKKILRVE
Saccharomyces cerevisiae MSK----------------------------------EDFVIKPEAAGASTDTSEWPLLLKNFDKLLVRSGHYTPIPAGSSPLKRDLKSYISSGVINLDKPSNPSSHEVVAWIKRILRCE
Kluyveromyces lactis MS-----------------------------------DEFVIKPESVSPSSNTSEWPLLLKDYDKLLVRSGHYTPIPAGASPLKRDLKSYISSGVINLDKPSNPSSHEVVAWIKRILRCE
Ashbya gossypii
MS-----------------------------------EEHMIKPENVAPSADTSEWPLLLKNYDKLLVRSGHYTPIPAGASPLKRDLKSYVSSGVINLDKPSNPSSHEVVAWIKRILRCE
Lodderomyces elongisporus MSK----------------------------------EDFQIKPEAVTPANNTSDWPLLLKNYDKLLVRSGHYTPIPAGSSPNNRDLKSYVSSGVINLDKPSNPSSHEVVAWIKRILRVE
Pichia stipitis
MS-----------------------------------DEYFIKPEAVSPSNDTSEWPLLLKNFDKLLVRSGHYTPIPSGSSPLKRDIKSYISSGVINLDKPSNPSSHEVVAWIKRILRSE
Pichia guilliermondii MAPHDAMRIF------------LNTIIVVFIFPVAMSDEYMIKPESIAPSNDTSEWPLLLKNFDKLLVRSGHYTPIPAGSSPLKRDLKSYVSSGVINLDKPSNPSSHEVVAWIKRILRTE
Zygosaccharomyces rouxii MSK-----------------------------------DFVIKPDTSGPTPDTAEWPLLLKDYDKLLMRSGHYTPIPAGSSPGKRDIKSYVNSGVINLDKPSNPSSHEVVAWIKRILRCE
Aspergillus fumigatus MAV----------------------------SVTKEEMDYTIKPEAGASNISTEDWPLLLKNYDKLMVRTGHFTPIPAGCSPLKRDLKSYISSGVINLDKPSNPSSHEVVAWMKRILRAE
Aspergillus clavatus MAV----------------------------ALAKEEMDYTIKPEAGASNISTEEWPLLLKNYDKLLVRTGHFTPIPAGCSPLKRDLKSYVSSGVINLDKPSNPSSHEVVAWMKRILRAE
Aspergillus nidulans M---------------------------------AKEVDYTIKPEATASNINTEDWPLLLKNYDKLMVRTGHFTPIPAGSSPLKRDLKSYINSGVINLDKPSNPSSHEVVAWMKRILRAE
Chaetomium globosum M----------------------SDSKALVKKGEDNEVEYMIKPQTLVPTLNTSDWPLLLKNYDKMLVRSGHFTPIPNGSSPYKRDIKSYVSSGVINLDKPSNPSSHEVVAWLKRMLRVE
Magnaporthe grisea MGG-------------------NKDALALARRDEEVDEEFSIKPEQVTPALDTSSWPLLLKNYDKLHIRTGHFTPIPNGSTPLRRDIKSYISSGVINLDKPSNPSSHEVVAWIKRILRCE
Neurospora crassa
MG---------------------------------AETEHVIKPQATEPAVDTSEWPLLLKNYSNLLVRTGHFTPIPAGSSPSKRDIKSYVSSGVINLDKPSNPSSHEVVAWIKRMLRVE
Botryotinia fuckeliana MALV--------------------------STKKEQEVDYTIKPEALTPAVDTSSWPLLLKNYNNLLVRTGHFTPIPNGCTPLKRDLKSYISSGVINLDKPSNPSSHEVVAWVKRMLRVE
Pyrenophora tritici-repentis MALV-----------------------------QTDPDAYVIKPAAAAPAIDTSDWPLLLKNYSDLLVRTSHYTPIPNGCAPLSRDLKSYISSGVINLDKPSNPSSHEVVSWIKRMLRVE
Neosartorya fischeri MAV----------------------------SVAKEEMDYTIKPEAGASNISTEEWPLLLKNYDKLMVRTGHFTPIPAGCSPLKRDLKSYISSGVINLDKPSNPSSHEVVAWMKRILRAE
Coccidioides immitis MAA------------------------------SKSDMDFSIKPEAATPPIPTSEWPLLLKNYDKLLVRTGHFTPIPAGCTPLKRDLKSYISSGVINLDKPSNPSSHEVVAWMKRILRVE
Dictyostelium discoideum MTT--------------------DKKSKSKSSEKSTQEVEQVIKPEKTPILDTSKWPLLLKNYDQLSVRTGHYTPIPNGHSPLKRPIKEYVKYGIINLDKPSNPSSHEVVAWIRTILRVT
Arabidopsis thaliana MAEVDI-------------SHSKKKKQDKTENDAADTGDYMIKPQSFTPAIDTSQWPILLKNYDRLNVRTGHYTPISAGHSPLKRPLQEYIRYGVINLDKPANPSSHEVVAWIKRILRVE
Chlamydomonas reinhardtii MSV----------------------------GEAQQRTDFLIQPEKTVPKLDTSKWPLLLKNYDKLNVRTGHYTPIPAGSTPLKRPLKEYLSYGCINLDKPANPSSHEVVAWVKRMLRVE
Cryptosporidium parvum SSM---------------------GKRKELEEMSDGKIETVIKPESKSQPIDTSNWPLLLKNYSKLNVRTGHYTPLPYGNSPLNRNLADYLSYGIINLDKPANPSSHEVVAWIKKILRCE
Euglena gracilis
MAKA----------------------------QWENDFSIPTESGEKGAALDSSEWPLLLKNYDKLNVRSAHYTPIPQGHTPLKRPIKAYLQYGCLNLDKPSNPSSHEVVSWLKRILKVE
Methanocaldococcus jannaschii MILLE------------------------------------------------KTQEKKINDKEELIVKEEVETNWDYGCNPYERKIEDLIKYGVVVVDKPRGPTSHEVSTWVKKILNLD
130
140
150
160
170
180
190
200
210
220
230
240
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
|---TruB II--|
Homo sapiens
KTGHSGTLDPKVTGCLIVCIERATRLVKSQQSAGKEYVGIVRLHNAIEG-GTQLSRALETLTGALFQRPPLIAAVKRQLRVRTIYESKMIEYDPERRLGIFWVSCEAGTYIRTLCVHLGL
Rattus norvegicus
KTGHSGTLDPKVTGCLIVCIERATRLVKSQQSAGKEYVGVVRLHNAIEG-TAQLSRALETLTGALFQRPPLIAAVKRQLRVRTIYESRVVEYDPERRLGVFWVSCEAGTYIRTLCVHLGL
Mus musculus
KTGHSGTLDPKVTGCLIVCIERATRLVKSQQSAGKEYVGIVRLHNAIEG-GTQLSRALETLTGALFQRPPLIAAVKRQLRVRTIYESKMIEYDPERRLGIFWVSCEAGTYIRTLCVHLGL
Bos taurus
KTGHSGTLDPKVTGCLIVCIERATRLVKSQQSAGKEYVGIVRLHNAIEG-GTQLSRALETLTGALFQRPPLIAAVKRQLRVRTIYESKMIEYDPERRLGIFWVSCEAGTYIRTLCVHLGL
Gallus gallus
KTGHSGTLDPKVTGCLIVCIERATRLVKSQQSAGKEYVGIVRLHNAIES-EAQLARAIETLTGALFQRPPLIAAVKRQLRVRTIYESKLVEYDPERRLGIFWVSCEAGTYIRTLCVHLGL
Danio rerio KTGHSGTLDPKVTGCLIVYVERATRLVKSQQSAGKEYVGIVRLHNAL