10 20
30 40
50 60 70
80 90 100 110 120
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
Tetrahymena thermophila ---------------------------------MQKINNINNNKQMLTRK---------------------EDLLTVLKQISALKYVSNLYEFLLATEKIVQTS---------ELDTQFQ
Paramecium tetraurelia -------------------------------------------------------------------------MLFTLKNIQQFQSVFTFQQYLLFIG---------------AEFDQNT
Paramecium caudatum -------------------------------------------------------------------------MLITLQQIAQLYNVITLQDYLKYLD---------------VDPPKN-
Euplotes aediculatus -------------------------------------MEVDVDNQADN-----------------------HGIHSALKTCEEIKEAKTLYSWIQKVIRCRNQSQS-----HYKDLEDIK
Oxytricha trifallax MSAKKPVQSKLNIGNPTIPVTSNRSTAPKPVPGQPQFVNQEKKQQSQNTTTGAFRSNQNNANSGGNGNFELDDLHLALKSCNEIGSAKTLFCFFMELQKINDKIPSKKNTELIKNDPQFQ
130
140
150
160
170
180
190
200
210
220
230
240
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
|-------------
Tetrahymena thermophila EFLTTTIIASEQNLVEN---YKQKYNQPNFSQLTIKQVIDDSIILLGNKQN-----YVQQIGTTTIGFYVEYENINLSRQ-----------------------TLYSSNFRNLLNIFGEE
Paramecium tetraurelia IFYQTTLICSDNIP-------LKKFPIIKPSNFLYQKLFEEKKKDEN-----------KCIQYSNNENNFQNKKYFLVSQK----------------------LILSDEMETFYKIVGEQ
Paramecium caudatum IFFQNTIICSETQN-------LQKFPIKKPLNFLYQKLSDDQRRDE-------------IVTDSRNDFQFQKQKSFIGCQK----------------------LMLSDNMEHLYREIGEQ
Euplotes aediculatus IFAQTNIVATPRDYNEEDFKVIARKEVFST--GLMIELIDKCLVELLSSSDVSDRQKLQCFGFQLKGNQLAKTHLLTALST-------------------QKQYFFQDEWNQVRAMIGNE
Oxytricha trifallax YFCHNTILCTEQSYDEKTIEKLAKLEYKLEYKNSYIDMISKVIKELLIENKLN---KLQTFGYKLVNNEFGNQNHLGMMQQNQDSSHNSNFMVKCDYINLNKQCMITKNWEKVYFYLGDH
|----------------
250
260
270
280
290
300
310
320
330
340
350
360
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
---Motif T2------------------| |----CP2-
Tetrahymena thermophila DFKYFLIDFLVFTK--VEQNGYLQVAGVCLNQYFSVQVKQKKWYKNNFNMNGKATSNNNQNNANLSNEKKQE------------------------------NQYIYPEIQRSQIFYCNH
Paramecium tetraurelia NIDFILQNFKILIKEDTNKETYIQIWGEPIMKNTIKQ-----------------------------------------------------------------KSNNQEKICRQQILYCNH
Paramecium caudatum NFDYLLKNYKILIKDDNDSETYIQISGDPIMKIQPKQ-----------------------------------------------------------------KQQIAEKIQRQQILYCNH
Euplotes aediculatus LFRHLYTKYLIFQR--TSEGTLVQFCGNNVFDHLKVN--------------DKFDKKQKGGAADMNEPRCCS------------TCKYNVKNEKDHFLNNINVPNWNNMKSRTRIFYCTH
Oxytricha trifallax LFMHIYKEYMIFLK--TRDESLVQISGTNIFCYLNEKLGRLQAAFYEGPNKNAANSAAQGSNPEANDLISAEQRKINTAIVMKKTHKYNIKAADESYLTNQEKGFWDDQIKRNRLFYCAH
---Motif N-------------------|
370
380
390
400
410
420
430
440
450
460
470
480
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
--| |------Motif CP-------------|
Tetrahymena thermophila MGREPGVFKSSFFNYSEIKKGFQFKVIQEKLQGRQFINSDKIKPDHPQTIIKKTLLKEYQSKNFSCQEERDLFLEFTEKIVQNFHNINFNYLLKKFCKLPENYQSLKSQVKQIVQSENKA
Paramecium tetraurelia MSRQVGFFKKNFISQAKKEIIMRNFIPKEQISN-----------EMISMKATSNIFDQCFFEGKFKEQEKQQLRILTQKLITNMQKFNFPQAFKIFVPLPTNYLNLKKEVSQSIK----Q
Paramecium caudatum MSRQVGMFKKNFISYAKKQIIKSNYIKMQQITP-----------EIIAIKSTSNILDYFYNNKQLSPIEISHLRPILESCMKKLQKFNFHGQFKFFVPLPSNYKQLKQETKRHLK----D
Euplotes aediculatus FNRNNQFFKKHEFVSN--------KNNISAMDRAQTIFTNIFRFNRIRKKLKDKVIEKIAY------------------MLEKVKDFNFNYYLTKSCPLPENWRERKQKIENLIN----K
Oxytricha trifallax QNR---FFQKHILNS----------KTLSQQQIRDNIYKEVFGFNRVRAELKGKVMSIIEQ------------------VIVNQKKFDFKYYLSKNCPLPENWKNLKKSFLEDAA----V
490
500
510
520
530
540
550
560
570
580
590
600
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
|------------
Tetrahymena thermophila NQQSCENLFNSLYDTEISYKQITNFLRQIIQNCVPNQLLGKKNFKVFLEKLYEFVQMKRFENQKVLDYICFMDVFDVEWFVDLKNQKFTQKRKYISDKRKILGDLIVFIINKIVIPVLRY
Paramecium tetraurelia NLYYIPNLYDEIYKNFLSYDQVISILRYFLRTIIPIDFLGQSNLYTFLKDLSQFITFLRFEDQNYLDYINRLNVFQIPWMNSYFSK--KKQKILISKKRQQLLPIFRFLFQLIIIPFLRH
Paramecium caudatum SLN-VSQLLDHLYLNHIPHQQITSLVRNFLREIIPIDLFGQQNLFTFLTDISDFITLLRFEDQSYLDYIKKMNVFEVPWLNKYFNK--KKQRLLIIKKRNQMIPLMKFMFQEIIIPFLRH
Euplotes aediculatus TREEKSKYYEELFSYTTDNKCVTQFINEFFYNILPKDFLTGRNRKNFQKKVKKYVELNKHELIHKNLLLEKINTREISWMQVETSA--KHFYYFDHENIYVLWKLLRWIFEDLVVSLIRC
Oxytricha trifallax SGELRGQVFRQLFEYQQDQRQISNFLTEFVANVFPKNFLEGKNKKIFNKKMLQFVKFNRFESFTKISLLNKFRVNEVSWLSFKCKD--ENKKFFMNENEHVFFKVLKWVFEDLAITLMRC
610
620
630
640
650
660
670
680
690
700
710
720
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
--------Motif T-------------------| |-Motif 1||-------Motif 2-----|
Tetrahymena thermophila NFYITEKHKEGSQIFYYRKPIWKLVSKLTIVKLEEENLEKVEEKLIPEDS-FQKYPQGKLRIIPKKGSFRPIMTFLRKDKQKNIKLNLNQILMDSQLVFRNLKD-----MLGQKIGYSVF
Paramecium tetraurelia NFYITERMKDDWKLFYYRKEIWTLVLKLSLNQLSSNNLKQISPEKITTQY------IGKLRIVPKPGTFRPIVTYNRKSRISKLSLNKKLLDIKYVLRNLR-----SQQLGFSVFGNPQI
Paramecium caudatum NFYITERMKDDWKLFYYRKEIWHLILQLSLTSLTKNNFEEISVNSIKTPY------VGKLRIVPKPGTFRPIVTYNRKAKTSKISLNRKLVDIKYILRNLR-----IQPLGYSVFGNPEV
Euplotes aediculatus FFYVTEQQKSYSKTYYYRKNIWDVIMKMSIADLKKETLAEVQEKEVEEWKKSLGFAPGKLRLIPKKTTFRPIMTFNKKIVNSD-RKTTKLTTNTKLLNSHLMLKTLKNRMFKDPFGFAVF
Oxytricha trifallax YFYSTEKAKEYQRIFYYRKNIWNMIMRLSIDDLLKQNLKQVEKKEMRIFCESQNFAPGKLRLIPKGDTFRPIMTFNRKIPNQVGKFQSRMTTNNKLQTAHMMLKNLKSKMFKHSFGFAVF
730
740
750
760
770
780
790
800
810
820
830
840
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
|--------Motif A---------|
Tetrahymena thermophila DNKQISEKFAQFIEKWKNKGRPQLYYVTLDIKKCYDSIDQMKLLNFFNQSDLIQDTYFINKYLLFQRNKRPLLQIQQTNNLNSAMEIEEEKINKKPFKMDNINFPYYFNLKERQIAYSLY
Paramecium tetraurelia FNRLEEFKKLWIKYQF-----PQTYFMSMDIHKCYDSIQLEFLLKFIEESNLIQSAYVINKYYLIIRNNR---------------------QSRGS-----KQMRELFNIFDRTCAIPIN
Paramecium caudatum FSRLEEFKKLWIKLQQ-----PKTYYITMDIQKCYDTIILQKLLQFIEESKQFSSIYAINKYHIVSRNNR---------------------MLKPS-----FSMKDLFNILDRTCAIPFN
Euplotes aediculatus NYDDVMKKYEEFVCKWKQVGQPKLFFATMDIEKCYDSVNREKLSTFLKTTKLLSSDFWIMTAQILKRKNN---------------------IVIDSKNFRKKEMKDYFRQKFQKIALEGG
Oxytricha trifallax NYDDIMKRYENFVQKWKQINSPKLYFVAMDIEKCYDNVDCERVVNFLQKSDLMDKEYFILNTFVLKRKNN---------------------IIVERSNFRKLPIKQYFRYKFQKIGIDGS
850
860
870
880
890
900
910
920
930
940
950
960
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
|--------Motif B'--------| |--Motif C--|
Tetrahymena thermophila DDDDQILQKGFKEIQS--DDRPFIVINQDKPRCITKDIIHNHLKHISQYNVISFNKVKFRQKRGIPQGLNISGVLCSFYFGKLEEEYTQFLKN--AEQVNGSINLLMRLTDDYLFISDSQ
Paramecium tetraurelia NP--QALQKGYIEYIQ--QNKLAIIANLGIQTTVTFSEFLLSIKELCQNNIVQFEDRYFIQTLGIPQGLNISGILCSFYIANIEKN---------LTRKLIGDTLIMRLTDDYCCLTFDK
Paramecium caudatum KP--QLLQKGYIEHIQ--KNKQTIIINQGFQNSVTFSEFLNSIKNICQNNIVQFENRYFRQTLGIPQGLNISGILCSFYLANLEQK---------LTNKILGDTLIMRLTDDYCCLAFSQ
Euplotes aediculatus QY--PTLFSVLENEQNDLNAKKTLIVEAKQRNYFKKDNLLQPVINICQYNYINFNGKFYKQTKGIPQGLCVSSILSSFYYATLEESSLGFLRDESMNPENPNVNLLMRLTDDYLLITTQE
Oxytricha trifallax SY--PTLFEILEDEFNDLNMKRTIIVEQEQRKKFPKNDLLQPVLKICQNNYVTFNKKQYKQMKGIPQGLCVSYILSSFYYANLEENALQFLRKESMDPEKPEINLLMRLTDDYLLMTTEK
970
980
990
1000
1010
1020
1030
1040
1050
1060
1070
1080
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
|----------Motif
D--------| |--Motif E--|
Tetrahymena thermophila QNALNLIVQLQNCANNNGFMFNDQKITTNFQFPQEDYN-----LEHFKISVQNECQWIGKSIDMNTLEIKS-IQKQTQQEINQTINVAISIKNLKSQLKNKLRSLFLNQLIDYFNPNINS
Paramecium tetraurelia QNLITIKNNFKEVEKQYSIHLNDDKTQHNIDQK------------------IVSFKWIGKIINLDKLTLKPAFAQEKDSKFSNQINVNLPSRINSYFFKAKLKSLMLNQFKFFFNPKIND
Paramecium caudatum DSSIKILNNFTQIEKQYQIRLNHDKTQHNIERS------------------DRYFKWIGKIIDIETLTLKPAFYLESDTKFQYQINVNLPTKVKTYYIKSKLKSLILNQFKFFFNSKLND
Euplotes aediculatus NNAVLFIEKLINVSRENGFKFNMKKLQTSFPLSPSKFAKYGMDSVEEQNIVQDYCDWIGISIDMKTLALMPNINLRIEG-ILCTLNLNMQTKKASMWLKKKLKSFLMNNITHYFRKTITT
Oxytricha trifallax NNAMLFIEKLYQLSLGNFFKFHMKKLKTNFALNLQKIG-CTNTTQDIDSINDDLFHWIGISIDIKTLNIIQNINIKKEG-ILCTLNVNMQTNESILWLKKKLKSFLMNNISFYFKSTINT
1090
1100
1110
1120
1130
1140
1150
1160
1170
1180
1190
1200
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
Tetrahymena thermophila FEGLCRQLYHHSKATVMKFYPFMTKLFQIDLKKSKQYSVQYGKENTNENFLKDILYYTVEDVCKILCYLQFEDEINSNIKEIFKNLYSWIMWDIIVSYLKKKKQFKGYLNKLLQKIRKSR
Paramecium tetraurelia QPTLIKIAKTFIHAGLIKLINFMEKAKL-FKVSK----------QKS--NKIDFLKIIKQVQIEIANYCFQQAQERGSELD--QNYILRIIKKQLQKTIKNHKKLKTIINYKNL------
Paramecium caudatum KPTMIKVLKIFVHSGLVKLISFLKRLKYNYGASK----------LRNQGKSIRLLKIIKEIKVEIAAYCVQQSRESNMEID--QEYLMRIIQKQLKKTIRNNSYLRKKINYKQI------
Euplotes aediculatus EDFANKTLNKLFISGGYKYMQCAKEYKDHFKKNL----------AMSSMIDLEVSKIIYSVTRAFFKYLVCNIKDTIFGEEHYPDFFLSTLKHFIEIFSTKKYIFNRVCMILKAKEAKLK
Oxytricha trifallax KQFANITLSKLYIAAAEKYVACCQEFKRFHENTS----------LGGQNIDIKIIHIIYVVIRSFFKYLVCNVKSPVFERDDYQQFFIYSLKFFITRFKQQKNEFAGVYKILKAKEKKLE
1210
1220
1230
1240
1250
....|....|....|....|....|....|....|....|....|....|....|....
structures
Tetrahymena thermophila FFYLKEGCKSLQLILSQQKYQLNKKELEAIEFIDLNNLIQDIKTLIPKISAKSNQQNTN
Paramecium tetraurelia -----------------------------------------------------------
Paramecium caudatum -----------------------------------------------------------
Euplotes aediculatus SDQCQSLIQYDA-----------------------------------------------
Oxytricha trifallax VAKIEFQIQ--------------------------------------------------