10 20
30 40
50 60 70
80 90 100 110 120
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
motif
Ciona intestinalis MKPVDWKIKTNCRAKFTSCEYVDIENSIKPMQHPSNMQSILPLHKIFYSKVSFNDKNNQDIFPTSHILMKLSKSKTGARDLVRHIFKDQNVENEIGKKRSRETDETLQEPVSKKRCVIDD
Ciona savignyi ------------------------------------MNLNIYVHTILYSKVSFNDKTNEEIFPRCHVLRKLSVSNTSARKLVQHIFKDIISESRTSVDKIIKKPAISQNLQLQKTSAVNT
Apis mellifera -------------------MMDNFTLQSLKKDFGTYFQQYCLHHKILIKKNKCNAFIVSNVCDLKKCIAAVNCLKFNINRKLKKLQTDALFINSLNPIYYIPKLDEQYKIQEKINRNDNQ
Bombyx mori ------------------------------------MNTPICFSKYFVDKYNLRSFDN--------VLKSKVFSYDDSTFCLYLLKSETSIS----------------------------
Bombyx mandarina ------------------------------------MNTPICFSKYFVDKYNLRSFDN--------VLKSKVFNYDDSTFCLYLLKSETSIS----------------------------
Tribolium castaneum
------------------------------------------------------------------------------------------------------------------------
Caenorhabditis elegans
------------------------------------MAPTIKSSLTTLHEEIR------------------KYDK---RRHWNTIIRKS-------------------------------
Caenorhabditis remanei
------------------------------------MSPEYRLLFKFQKKYVIS---------------LAKLHKNLGENHWSTILREN-------------------------------
region
130
140
150
160
170
180
190
200
210
220
230
240
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
motif
Ciona intestinalis TDN-ATLKSELETEFVGSKLKQTDVVPMVKQANEKVLINKNDSSVAKSLFSSSFAKVFSMAHTIKLETLSHEFDSSLPSTADASDSKPTKRS----KFKAKRKQKPNLPRRLRKCIPLCY
Ciona savignyi TNFTDTSATLFEKTFAG---------------------FGFETKVKKKHVAFNGCNKLCKASSTYKENLS-KFQLAFTPDKIRIISEPE-------VFKTS-KDKLNILRRLRKCIPLCY
Apis mellifera DKQYKIKKK-------------------------------------------KNFHKEITYHNIFLNNLNLNIFLCNTSQILPYYTNYKSFSICILNIDKKGHEIYDFITKGNRKDAIAI
Bombyx mori ----------------------------------------------------SLTKELIQIFSLMKSNLKSKCKEDFTQYFNQLTQKVG--------LEEVDKEFIFKCSYQALCDIVPF
Bombyx mandarina ----------------------------------------------------SLTKELIKIFSLMKSNLKNKCKEDFTQYFNQLTQKVG--------LEEVDKEFIFKCSYQALCDIXPF
Tribolium castaneum
------------------------------------------------------MVHYYRLSLKSRQKAPKIVNSKYNSILNIALKN-----------------FRLCKKHKTKKPVQIL
Caenorhabditis elegans
-------------------------------------------------------AHLSLVRNIFKKKRRQKLNYRHMSTFLASSENIS---------NIRDTVVLLEINKLSQSILSSR
Caenorhabditis remanei
-------------------------------------------------------ANLAILRNIFKRKMKGKLNYRHMSAFLVSSENVL---------KVRNTVVMDEVDKLSKSIVSTR
region
|------------------re
250
260
270
280
290
300
310
320 330 340 350 360
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
motif
|-----------motif CP--------| |-------------------------------motif
QFP------------------------------
Ciona intestinalis NILKKTDNRKLFSCLSHYTKFISPFERSFAKKNLDLLMEISTIEL---TEHISKMLQHYTSTHQVFLYLRSALKKTFPIHLMWGTKHNRSVFLKNLEKFLSCRRFDKVTIADFTRGIVME
Ciona savignyi NLLKKTNNRTIFNKLTYYTKFKSSGERSLTKQDLHQLSKLPVTQL---TEHLSKMLQLYTTKHQVYLFLRASLKKTIPIHLMWGTKRNQTAFHKNLKQFLGCRRYDKFSILDFTKGLCIE
Apis mellifera NYEDYFLLIEKFQNRHKKFNYYSVLKHLICKQKYNEKTKCIYEINKKQLKNFFDLIFAKVVPKRLFGSITNLKKIKRATFQLLDTPCFKYFNLKPIIEKLDIINITWLQNVTNVKTKWLV
Bombyx mori NFFGNYENKKLFRCMVHDIIFSMKMQHLLPE------------------KYLKTWDFDILPWRNISYTILHKILLWLLTAILPSIICLTFYVTTCKLDSDENKLI---------------
Bombyx mandarina NFFGNYENKKLFRCMVHDIIFSMKMQHLLPG------------------KYLKTWDFDILPWRNISFNILHKILLWLLTAILPSIICLTFYVTTCKLDSDENKLI---------------
Tribolium castaneum
ALLQEIIPKSYFGTTTNLKRFYKVVEKILTQSSF---------------ECIHLSVLHKCYDYDAIPWLQNVEPNLRPKLLLKHNLFLLDNIVKPII-----------------------
Caenorhabditis elegans
NIFELRKLKPTRKMVEIEHCFVSKNDTFVACSKLIS-------------MFHLEKYIGSTNCANILEWITKMLNNGGYHKMKPFVYKEAPFTRAFRASKVMKNVDPNVKRNIQTSIV---
Caenorhabditis remanei
HNFELRKLAPKRKKKNSRDCYMFEHETMNIIRRVFT-------------IFRLRQHIGSTNCDSMLVWIMNIMKQEKLQYVPDFVFKEAPLARAFRAKQVLKDVDPLSKRYSQFSII---
region
gion v-II--------------|
|--------------------------------------region v-III-
370
380
390
400
410
420
430
440
450
460
470
480
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
motif
----|
|-------------------motif T------------------------|
|-motif 1| |-2-|
Ciona intestinalis DLPWLKGEDTSSNLLRFFQWMIEDYVFVVLSGSFYITENSNFKNRLFFYHKKMWAEIVAYGVALLLKQNRWAVPPIADVTIRPYFSVRFCPKVNG-LRPIMRLRNADKM----PDMVPFE
Ciona savignyi DLPWVKGIYGKDTLLQFFKWIIEEYLFVLIRGSFYITDNGNFKNRLFFYPKKIWSEAVAYGVALLLKQKRWAFHSSEKHTVPPYFAVRFCPKLNG-LRPIMKLRNPKEP----SSKMSFN
Apis mellifera MA-------------QFVKWFFTEFLFNILYTYFHITKVSGNTNERLYITRFTWNNIQKKFIKEGIHSNTLQPNIKRNTWNPSIGIYKLLCKYSD-VRPIFKLKKK------FRDTDYLF
Bombyx mori ---------------YFWKSQWQSFCDKKISKMLFSNVMEKFEPYCLGKKIKRNLPIAKRLDLKKLIKEVP--------------KLHLVLKPNYDCRPIVKYRNDELNKRQKYRIKEKL
Bombyx mandarina ---------------YFWKSQWQSFCDKKISKMLFSNVMEKFEPYCLGKKVKRNLPIAKRLDLKKLIKEVP--------------KLHLVLKPNYDCRPIVKYRNDELNKRQKYRIKEKL
Tribolium castaneum
---------------AFYYKPIKTLNGHEIKFIRKEEYISFESKVFHKLKKMKYLVEVQDEVKPRG-------------------VLNIIPKQDN-FRAIVSIFP----------DSARK
Caenorhabditis elegans
---------------NNLKSGVHWWALMALRQVMIPIVIKEERVLLWRDGYLNILTKEIKDFKQRYIVQKAPH-----FIRPNVATFKLSISRQK-LRPLFKRKA--------IDKKTET
Caenorhabditis remanei
---------------NHLRGALIWWIFAALRQIMIPIDVKRKKVYLWRGGYLKLLRREMKDFKERYKVKRKPAFVLNPSPNSVVGRLKFDIVENK-FRPIIRRNP--------IDKVKDK
region
---------------------------| |-------------------region
v-IV------------------|
490
500
510
520
530
540
550
560
570
580
590
600
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
motif
|---------motif
A------------|
Ciona intestinalis VIASVLKLVCSKVPQATGFATQHHAHIYNGWKLFVLSLAGIP-GRKRLFFVKMDIHRCFDSIPVVKLILIVEGLLDRSGEVDLTSATKGKLN----------------------------
Ciona savignyi SISSVLKLALQRFPDSLGFAIKDRSDIYCKWRQFLLKLKEINAKRWQLYFVKLDICRCFDSIPIPKLLRIVQDLLDRAGTLDVSIVTKGKLR----------------------------
Apis mellifera IIFKFLKQLYVTNYEVINFPKKWKSIVQKLNSKT-----------EKLYFVCCDVINAFGSIIQVELYNIIQSLCKDLPENLILKYYTMKSE---ISKKDNTYHKYFSDLQLPFPSDQFY
Bombyx mori QLLKLLTGNQQHKIETAYPKLHSKWLELNK---------------PKLYFIKTDLSDAFGSINTDKMMKILHENYLNYHKTES-------------------------------------
Bombyx mandarina QLLKLLTGNQHHKIETAYPKLHSKWLELNK---------------PKLYFIKTDLSNAFGSINTDKMMKILHENHLNYHKTES-------------------------------------
Tribolium castaneum
PFFKLLTSKIYKVLEEKYKTSGSLYTCWSEFTQKT---------QGQIYGIKVDIRDAYGNVKIPVLCKLIQSIP---------------------------------------------
Caenorhabditis elegans
MQWKRLNSMLSWCLERSGVYRHTIRDSCKKVSDFLKKNS----QNSKIIGYTADVSKCFSTVNHDVLISIIDRLFSQEHDIYTVCGKGRNHGGFHKLIFCSAGTELNAHEALRRKMELKG
Caenorhabditis remanei
MHWKKVNSMLTWCLEKGGETRQSINSSCKILLKFLRRND----TFPKLFGYTADVSKCFSAVKHSTLTSIIERLLDIW-----TACGKGRDKKGFHKLIFCSNDSEEGAYQSLKKKMESK
region
610
620
630
640
650
660
670
680
690
700
710
720
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
motif
|-------------motif
B------------| |----motif
C----| |----------motif D-----
Ciona intestinalis -------CKVKHCTHCILTTLKYFLNKVTLSVLSKKFVKLHGIPQGWELSNLLCDIFYGHMEQQHLINVLDPIDGEPHLILRYVDDYLFVTTSRYK-ANAFLKLMYSDNFKEYGFIVNTK
Ciona savignyi -------CKIHHCSQYIVDQLKFFLRDVTLSVLSKTFIKLNGIPQGWLLSSLLCNIFYGHMEQTHLFKVFEGINHEPCLLLRYVDDYLFITTNQDK-ATTFLQLMHSG-FEDYGCKINPK
Apis mellifera ANLVDNQWYQLINKSWLLESISKYIFYQRIKINKNIHIIGKGIVQGSMLSPILSDIYYNYILNKEMSTYLKTGE-----IIKYMDDILYITENKTL-AEQFLELTKKG-IPQYNCYFKQS
Bombyx mori -----NMVLKKRVSEQFRNLVAELRKPLLVRFGSTIFRWKEGLVQGYKYSPALSELYYSYMDKKYLTEHLKVDNRKIKMFFRVVDDYLYITDCFDD-AIAFLSKISNY------RNVNYS
Bombyx mandarina -----NMVLKKRVSEQFRNLVAELRKPLLVRFGSTIFRWKEGLVQGYKYSPALSELYYSYMDKIYLTEHLKVDNRNIKMFFRVVDDYLYITDCFDD-AKAFLSKILNY------RNVNYS
Tribolium castaneum
---------THLLDSEKKNFIVDHISNQFVAFRRKIYKWNHGLLQGDPLSGCLCELYMAFMDRLYFSNLDKD-----AFIHRTVDDYFFCSPHPHKVYDFELLIKGVY-------QVNPT
Caenorhabditis elegans
VFNFEVCYREMSSSTTLYSVIRTTLSTYYYKRGPTSWRITKGVPQGHPISSNLAHMYLNNFEQKYWSNEKED---SRIVFCRYEDDFIFITTENSL-FEKMMKPLSTGNNTHF-----LT
Caenorhabditis remanei
HVEDHTVIYCNVCSKWLLDQLRSTISSYCYKRGKTTFKITKGVPQGHPLSPNFAFMYLNDFEKENWRKVEHD---ARIIYCRYVDDYVFMMTDKDL-FKQISQPLFTGKNQHGVKANYEK
region
730
740
750
760
770
780
790
800
810
820
830
840
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
motif
-----|
|---motif E---||-----------------------------------------------motif
E-I----------------------------
Ciona intestinalis KTCTNFAS-DCSQQLEDLQEQDTFPWFGYLFPTCL-----NMGRKQLKQKGTHNFGGVSVDLSAYQSTHCIRDFISVQSPGTYNYFQQIRRNLTRRVTEICFDPQIN-------GKMRIY
Ciona savignyi KTCINFHPNFIDSQVLILNNEEPFHWFGYIFPTCI-----NMNKKQIKQTGTNNFGGISMDLSSYQSTRRYRDFMSTRSSVTIYRQCSIQKNMTRRVDHMLFDPQIT-------CRKRIF
Apis mellifera KTQSNLIS------DRNKIVKKNIIYIGYKINCTT--------------------LEIELKRSDTALCHSISFLKPSKLSLLKHLSMRLSNVATFRLSKVMLDHTIN-------SKKTIM
Bombyx mori KTAVNFQHPSIRY-------SEDITFIGYSYNTVTLEVSRVNSIFYGQICYKINFTSAVENISKFIENRIGQSGIQISSHLFNLQYNSEELIWKHVFTTFCFSGNKFCTILAIACCEKDM
Bombyx mandarina KTAINFQHPSIQY-------SENITFIGYSYNTVTLEVSRVNSIFYGQICYKIKFTNAIENISNFIENRIGQSSIQISSHLFNLQYNSEELIWKHVFTTFCFSGNKFCTILAIACCEKDM
Tribolium castaneum
KTRTNLPTHRHP--------QDEIPYCGKIFNLTT--------RQVRTLYKLPPNYEIRHKFKLWNFNNQISDDNPARFLQKAMDFPFICNSFTKFEFNTVFNDQRT-------VFANFY
Caenorhabditis elegans
ANPKKFKKSERCGA------SQVLQWCGVKLDFQS---------------------------------------------------------------GNCFIRRR--------CKDGVA
Caenorhabditis remanei
CKVLTFQES-----------DDKLIWCGVKMDLKN---------------------------------------------------------------AKFYRRKR--------CLDGTI
region
|-----region
v-V-----|
|-----------------------region v-VI-----
850
860
870
880
890
900
910
920
930
940
950 960
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
motif
------------------------|
|------motif E-II--------|
|---------motif
E-III-----------|
|
Ciona intestinalis RNLY----ELAMLGAIQMVAHQLHEGQPWVIGNPWQFRSNIKQMANAVLARIQRVFKSKHFLTRPLVSWLFYKAFKTKMKRHSNNKAALPVLQMGINQCVHQLCEVGEWDNVRHYLQRAV
Ciona savignyi RNLY----EIALLGAIHTVAYDLHRKD--IYKDPLIINIFINLVAHRVWVIIQRKFKGKHFLSNSLVKWIFFKAFLVKLKRHHYYKKVIPSLHRQVLLNTTRLQNSKKWRENRFKMREAL
Apis mellifera RILK----EVCVLQANRA-------------------CILIKDLFDNVHNHIQNIFKIIKSTNEKITRYIIRMFLISQQKTSKLKIYKWNNQILHILWTSYKKVFMKDNILRQYFITFFS
Bombyx mori KNYLPLYKKRVTVRITNAILANLMMAKPKLIEFVYCINHFRYLSWLALYYNAQKTWKCSNLIPLIKIELAKSNCLFGKWREHARFISSNGDVQNHAIKEICRRSDLRAIVKKMDRLPYGF
Bombyx mandarina KNYLPLYKKRVTVRITNAILENLMMAKPKLIEFVYCINHFRYLSWLALYYNAQKTWKCSNLIPLIKVELAKSNCLFGKWREHARFISSNGDVQNTAIREICRRSDLRAIVKKMDRLPYGF
Tribolium castaneum
DAMICVAYKFDAAMMALRTSFLVNDFGFIWLVLSSTVRAYASRAFKKIVTYKGGKYRKVTFQCLKSIAWRAFLAVLKRRTEIYKGLIDRIKSREKLTMKFHDGEVDASYFCKLPEKFRFV
Caenorhabditis elegans
RQFL--------IKLQ--------------------------------------------------------------------------------------------------------
Caenorhabditis remanei
RDHLI-------------------------------------------------------------------------------------------------------------------
region
-------------------------------------------|
|-----------------------------------------region
v-VII--------------------
970
....|....|....
motif
E-IV|
Ciona intestinalis GMFPIHYRKVNCGL
Ciona savignyi INFPVTLCKLKT--
Apis mellifera --------------
Bombyx mori EVYNKKKF------
Bombyx mandarina EVYNKKKF------
Tribolium castaneum
KINRKASI------
Caenorhabditis elegans
--------------
Caenorhabditis remanei
--------------
region
-------------|