10 20 30 40 50 60 70 80 90 100 110 120

....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|

           |-NL--|                                                                            |---TruB I---|            

Homo sapiens MADAEVII-LPKKHKKKKERK---SLPEEDVAEIQHAEEFLIKPESKVAKLDTSQWPLLLKNFDKLNVRTTHYTPLACGSNPLKREIGDYIRTGFINLDKPSNPSSHEVVAWIRRILRVE

Rattus norvegicus MADAEAAMTFPKKHKKKKERK---PLPEADVAEIQHAEDFLIKPESKAAQLDTSQWPLLLKNFDRLNVRTTHYTPIPCGSNPLKREIGEYVRTGFINLDKPSNPSSHEVVAWIRRILRVE

Mus musculus MADAEVI-TFPKKHKKKKDRK---PLQEDDVAEIQHAEEFLIKPESKVAQLDTSQWPLLLKNFDKLNVRTAHYTPLPCGSNPLKREIGDYIRTGFINLDKPSNPSSHEVVAWIRRILRVE

Bos taurus MADAEVLI-LPKKHKKKKERK---SLPEEAVAEIQHAEEFLIKPESRVAQLDTSQWPLLLKNFDKLNVRTTHYTPLPCGSNPLKREIGDYIRTGFINLDKPSNPSSHEVVAWIRRILRVE

Gallus gallus MADGDG---SSVKKRRKKDKR---SLPDEDVADIQHTEEFLIKPESRVAQLDTSQWPLLLKNFDKLNVLTTHYTPLPSGANPLKREISDYVRSGFINLDKPSNPSSHEVVAWIRRILRVE

Danio rerio MADTES-----KKEKKRKSKK----ISDEEVGDIQESGDFLIKPESKVASLDTSQWPLLLKNFDKLNIRTAHYTPLPHGSNPLKRGIHDYVRSGFINLDKPANPSSHEVVAWIKRILRVE

Drosophila melanogaster MADVEV-----RKEKKKKKIKEEP-LDGDDIGTLQKQGNFQIKPSSKIAELDTSQWPLLLKNFDKLNIRSNHYTPLAHGSSPLNRDIKEYMKTGFINLDKPSNPSSHEVVAWIKKILKVE

Anopheles gambiae MEVDI-PTSPIKKEKKKKKIKQEPDTEIENLGEIQKSGDFQLKPSSAIASLDTSKWPLLLKNFDRLNVRTNHYTPLPFGSSPLNRKINDYVSAGFINLDKPSNPSSHEVVAWIKRILKVN

Aedes aegypti MSDYEMGSPTIKKEKKKKKIKQEPGAEIENLGEIQKSGNFQIQPSAAIAKLDTSQWPLLLKNFDRLNVRTNHYTPLPFGSSPLNRKIKDYVTSGFINLDKPSNPSSHEVVAWIKKILKVD

Culex pipiens quinquefasciatus MG-----SPHIKKEKKKKKIKQEPGVEVENLGEIQKSGDFQIKPSEAIAKLETSKWPLLLKNFDRLNVRTNHYTPLPFGSSPLNRKIKDYVTSGFINLDKPSNPSSHEVVAWIKKILKVE

Caenorhabditis elegans MG---------KKDKRSK-------LEGDDLAEAQQKGSFQLPSSNETAKLDASQWPLLLKNYDKLNVRTNHYTPHVEGVSPLKRDIKNYISSGFFNLDKPSNPSSHEVVSWIKRILRCE

Schizosaccharomyces pombe MTD-----------------------------THPGVDFMIKPEATSASKIDTAEWPLLLKNFDKLLVRTGHYTPIPCGNNPLKRPIAEYVSSGVINLDKPANPSSHEVVAWVKKILRVE

Saccharomyces cerevisiae MSK----------------------------------EDFVIKPEAAGASTDTSEWPLLLKNFDKLLVRSGHYTPIPAGSSPLKRDLKSYISSGVINLDKPSNPSSHEVVAWIKRILRCE

Kluyveromyces lactis MS-----------------------------------DEFVIKPESVSPSSNTSEWPLLLKDYDKLLVRSGHYTPIPAGASPLKRDLKSYISSGVINLDKPSNPSSHEVVAWIKRILRCE

Ashbya gossypii MS-----------------------------------EEHMIKPENVAPSADTSEWPLLLKNYDKLLVRSGHYTPIPAGASPLKRDLKSYVSSGVINLDKPSNPSSHEVVAWIKRILRCE

Lodderomyces elongisporus MSK----------------------------------EDFQIKPEAVTPANNTSDWPLLLKNYDKLLVRSGHYTPIPAGSSPNNRDLKSYVSSGVINLDKPSNPSSHEVVAWIKRILRVE

Pichia stipitis MS-----------------------------------DEYFIKPEAVSPSNDTSEWPLLLKNFDKLLVRSGHYTPIPSGSSPLKRDIKSYISSGVINLDKPSNPSSHEVVAWIKRILRSE

Pichia guilliermondii MAPHDAMRIF------------LNTIIVVFIFPVAMSDEYMIKPESIAPSNDTSEWPLLLKNFDKLLVRSGHYTPIPAGSSPLKRDLKSYVSSGVINLDKPSNPSSHEVVAWIKRILRTE

Zygosaccharomyces rouxii MSK-----------------------------------DFVIKPDTSGPTPDTAEWPLLLKDYDKLLMRSGHYTPIPAGSSPGKRDIKSYVNSGVINLDKPSNPSSHEVVAWIKRILRCE

Aspergillus fumigatus MAV----------------------------SVTKEEMDYTIKPEAGASNISTEDWPLLLKNYDKLMVRTGHFTPIPAGCSPLKRDLKSYISSGVINLDKPSNPSSHEVVAWMKRILRAE

Aspergillus clavatus MAV----------------------------ALAKEEMDYTIKPEAGASNISTEEWPLLLKNYDKLLVRTGHFTPIPAGCSPLKRDLKSYVSSGVINLDKPSNPSSHEVVAWMKRILRAE

Aspergillus nidulans M---------------------------------AKEVDYTIKPEATASNINTEDWPLLLKNYDKLMVRTGHFTPIPAGSSPLKRDLKSYINSGVINLDKPSNPSSHEVVAWMKRILRAE

Chaetomium globosum M----------------------SDSKALVKKGEDNEVEYMIKPQTLVPTLNTSDWPLLLKNYDKMLVRSGHFTPIPNGSSPYKRDIKSYVSSGVINLDKPSNPSSHEVVAWLKRMLRVE

Magnaporthe grisea MGG-------------------NKDALALARRDEEVDEEFSIKPEQVTPALDTSSWPLLLKNYDKLHIRTGHFTPIPNGSTPLRRDIKSYISSGVINLDKPSNPSSHEVVAWIKRILRCE

Neurospora crassa MG---------------------------------AETEHVIKPQATEPAVDTSEWPLLLKNYSNLLVRTGHFTPIPAGSSPSKRDIKSYVSSGVINLDKPSNPSSHEVVAWIKRMLRVE

Botryotinia fuckeliana MALV--------------------------STKKEQEVDYTIKPEALTPAVDTSSWPLLLKNYNNLLVRTGHFTPIPNGCTPLKRDLKSYISSGVINLDKPSNPSSHEVVAWVKRMLRVE

Pyrenophora tritici-repentis MALV-----------------------------QTDPDAYVIKPAAAAPAIDTSDWPLLLKNYSDLLVRTSHYTPIPNGCAPLSRDLKSYISSGVINLDKPSNPSSHEVVSWIKRMLRVE

Neosartorya fischeri MAV----------------------------SVAKEEMDYTIKPEAGASNISTEEWPLLLKNYDKLMVRTGHFTPIPAGCSPLKRDLKSYISSGVINLDKPSNPSSHEVVAWMKRILRAE

Coccidioides immitis MAA------------------------------SKSDMDFSIKPEAATPPIPTSEWPLLLKNYDKLLVRTGHFTPIPAGCTPLKRDLKSYISSGVINLDKPSNPSSHEVVAWMKRILRVE

Dictyostelium discoideum MTT--------------------DKKSKSKSSEKSTQEVEQVIKPEKTPILDTSKWPLLLKNYDQLSVRTGHYTPIPNGHSPLKRPIKEYVKYGIINLDKPSNPSSHEVVAWIRTILRVT

Arabidopsis thaliana MAEVDI-------------SHSKKKKQDKTENDAADTGDYMIKPQSFTPAIDTSQWPILLKNYDRLNVRTGHYTPISAGHSPLKRPLQEYIRYGVINLDKPANPSSHEVVAWIKRILRVE

Chlamydomonas reinhardtii MSV----------------------------GEAQQRTDFLIQPEKTVPKLDTSKWPLLLKNYDKLNVRTGHYTPIPAGSTPLKRPLKEYLSYGCINLDKPANPSSHEVVAWVKRMLRVE

Cryptosporidium parvum SSM---------------------GKRKELEEMSDGKIETVIKPESKSQPIDTSNWPLLLKNYSKLNVRTGHYTPLPYGNSPLNRNLADYLSYGIINLDKPANPSSHEVVAWIKKILRCE

Euglena gracilis MAKA----------------------------QWENDFSIPTESGEKGAALDSSEWPLLLKNYDKLNVRSAHYTPIPQGHTPLKRPIKAYLQYGCLNLDKPSNPSSHEVVSWLKRILKVE

Methanocaldococcus jannaschii MILLE------------------------------------------------KTQEKKINDKEELIVKEEVETNWDYGCNPYERKIEDLIKYGVVVVDKPRGPTSHEVSTWVKKILNLD

130 140 150 160 170 180 190 200 210 220 230 240

....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|

    |---TruB II--|                                                                                                      

Homo sapiens KTGHSGTLDPKVTGCLIVCIERATRLVKSQQSAGKEYVGIVRLHNAIEG-GTQLSRALETLTGALFQRPPLIAAVKRQLRVRTIYESKMIEYDPERRLGIFWVSCEAGTYIRTLCVHLGL

Rattus norvegicus KTGHSGTLDPKVTGCLIVCIERATRLVKSQQSAGKEYVGVVRLHNAIEG-TAQLSRALETLTGALFQRPPLIAAVKRQLRVRTIYESRVVEYDPERRLGVFWVSCEAGTYIRTLCVHLGL

Mus musculus KTGHSGTLDPKVTGCLIVCIERATRLVKSQQSAGKEYVGIVRLHNAIEG-GTQLSRALETLTGALFQRPPLIAAVKRQLRVRTIYESKMIEYDPERRLGIFWVSCEAGTYIRTLCVHLGL

Bos taurus KTGHSGTLDPKVTGCLIVCIERATRLVKSQQSAGKEYVGIVRLHNAIEG-GTQLSRALETLTGALFQRPPLIAAVKRQLRVRTIYESKMIEYDPERRLGIFWVSCEAGTYIRTLCVHLGL

Gallus gallus KTGHSGTLDPKVTGCLIVCIERATRLVKSQQSAGKEYVGIVRLHNAIES-EAQLARAIETLTGALFQRPPLIAAVKRQLRVRTIYESKLVEYDPERRLGIFWVSCEAGTYIRTLCVHLGL

Danio rerio KTGHSGTLDPKVTGCLIVYVERATRLVKSQQSAGKEYVGIVRLHNALEN-EHQLARALECLTGALFQRPPLIAAVKRQLRVRTIYESKLIEYDPERRLGIFWVSCEAGTYIRTLCVHLGL

Drosophila melanogaster KTGHSGTLDPKVTGCLIVCIDRATRLVKSQQSAGKEYVAIFKLHGAVES-VAKVRQGLEKLRGALFQRPPLISAVKRQLRVRTVYDSKLLDYDETRNMGVFWVSCEAGSYIRTMCVHLGL

Anopheles gambiae KTGHSGTLDPKVTGCLIVCINRATRLVKSQQSAGKEYVAVFKLHSAVEK-IAKVTQGLEKLRGALFQRPPLISAVKRQLRVRTVYDSKLLDYNDQRNIGVFWVSCEAGSYIRTMCVHLGL

Aedes aegypti KTGHSGTLDPKVTGCLIVCIDRATRLVKSQQSAGKEYVAVFKLHSAVES-VAKVNQGLEKLRGALFQRPPLISAVKRQLRVRTVYDSKLLDYDDQRNMGVFWVSCEAGSYIRTMCVHLGL

Culex pipiens quinquefasciatus KTGHSGTLDPKVTGCLIVCIDRATRLVKSQQSAGKEYVAVFKLHSAVES-VAKVNQGLEKLRGALFQRPPLISAVKRQLRVRTVYDSKLLDYDDQRNMGVFWVSCEAGSYIRTMCVHLGL

Caenorhabditis elegans KTGHSGTLDPKVSGCLIVCIDRTTRLAKSQQGAGKEYICIFKLHEEVED-DRKVKQALEKLTGALFQRPPLISAVKRQLRIRTVYENKFIEYDPAQQMGIFNCICESGTYVRTICVHLGL

Schizosaccharomyces pombe KTGHSGTLDPKVTGCLIICNDRATRLVKSQQSAGKEYVCVLRLHDSVEG-ERNVASAIETLTGALFQRPPLISAVKRQLRIRSIYESKLIEFDNERNLAVFWASCEAGTYMRTLCVHLGL

Saccharomyces cerevisiae KTGHSGTLDPKVTGCLIVCIDRATRLVKSQQGAGKEYVCIVRLHDALKD-EKDLGRSLENLTGALFQRPPLISAVKRQLRVRTIYESNLIEFDNKRNLGVFWASCEAGTYMRTLCVHLGM

Kluyveromyces lactis KTGHSGTLDPKVTGCLIVCVDRATRLVKSQQGAGKEYVCIVRLHDALKD-EKELGRGLENLTGALFQRPPLISAVKRQLRVRTIYDSNLIEFDNKRNLGVFWASCEAGTYMRTLCVHLGM

Ashbya gossypii KTGHSGTLDPKVTGCLIVCIDRATRLVKSQQGAGKEYVCIVRLHDALKD-EKDLGRELENLTGALFQRPPLISAVKRQLRVRTIYDSKLIEFDNKRNLGVFWASCEAGTYMRTLCVHLGM

Lodderomyces elongisporus KTGHSGTLDPKVTGCLIVCIDRATRLVKSQQGAGKEYVCIVRLHDQLEE-PKELNRALENLTGALFQRPPLISAVKRQLRVRTVYDSKLIEFDNKRGLGVFWASCEAGTYMRTLCVHLGM

Pichia stipitis KTGHSGTLDPKVTGCLIVCIDRATRLVKSQQGAGKEYVCIVRLHEELKD-AKDLGRSLENLTGALFQRPPLISAVKRQLRVRTIYDSNLIEFDNKRGLGVFWASCEAGTYMRTLCVHLGM

Pichia guilliermondii KTGHSGTLDPKVTGCLIVCIDRATRLVKSQQGAGKEYVCIVRLHDELKD-AKDMGRSLENLTGALFQRPPLISAVKRQLRVRTIYDSNLIEFDNKRGLGVFWASCEAGTYMRTLCVHLGM

Zygosaccharomyces rouxii KTGHSGTLDPKVTGCLIVCVDRATRLVKSQQGAGKEYVCIVRLHDALKD-EKDLGRSLESLTGALFQRPPLISAVKRQLRVRTIYDSNLIEFDNKRNLGVFWASCEAGTYMRTLCVHLGM

Aspergillus fumigatus KTGHSGTLDPKVTGCLIVCIDRATRLVKSQQGAGKEYVCVIRLHDKIPGGEAQFKRALETLTGALFQRPPLISAVKRQLRIRTIHESKLYEFDNDRHLGVFWVSCEAGTYIRTLCVHLGL

Aspergillus clavatus KTGHSGTLDPKVTGCLIVCIDRATRLVKSQQGAGKEYVCVIRLHDKIPGGEAQFKRALETLTGALFQRPPLISAVKRQLRIRTIHESKLYEFDNERHLGVFWVSCEAGTYIRTLCVHLGL

Aspergillus nidulans KTGHSGTLDPKVTGCLIVCIDRATRLVKSQQGAGKEYVCVIRLHDKIPGGEAQFKRALETLTGALFQRPPLISAVKRQLRIRTIHESKLYEFDNERHLGVFWVSCEAGTYIRTLCVHLGL

Chaetomium globosum KTGHSGTLDPKVTGCLIVCIDRATRLVKAQQGAGKEYVAVVRFHDTIPGGEPAFAKALETLTGALFQRPPLISAVKRQLRIRTIHESKLIEFDNDRHLGVFWVSCEAGTYIRTLCVHLGL

Magnaporthe grisea KTGHSGTLDPKVTGCLIVCIDRATRLVKSQQGAGKEYVCVIRLHDKVPGGEPEFARALETLTGALFQRPPLISAVKRQLRIRTIHESKLIEFDNDRHLGVFWVSCEAGTYIRTLCVHLGL

Neurospora crassa KTGHSGTLDPKVTGCLIVCVDRATRLVKAQQGAGKEYVCVIRFHDSVPGGEAAFASALETLTGALFQRPPLISAVKRQLRIRSIYESKLIEFDNDRHLGVFWVSCEAGTYIRTLCVHLGL

Botryotinia fuckeliana KTGHSGTLDPKVTGCLIVCIDRATRLVKSQQGAGKEYVCVIRLHDKLPGGEAQFARALETLTGALFQRPPLISAVKRQLRIRTIHESKLYEFDNDRHLGVFWVSCEAGTYIRTLCVHLGL

Pyrenophora tritici-repentis KTGHSGTLDPKVTGCLIVCIDRATRLVKSQQGAGKEYVCVIRLHDKLPGGEAQFARALETLTGALFQRPPLISAVKRQLRIRTIHESKLYEFDNERQLGVFWVSCEAGTYIRTLCVHLGL

Neosartorya fischeri KTGHSGTLDPKVTGCLIVCIDRATRLVKSQQGAGKEYVCVIRLHDKIPGGEAQFKRALETLTGALFQRPPLISAVKRQLRIRTIHESKLYEFDNDRHLGVFWVSCEAGTYIRTLCVHLGL

Coccidioides immitis KTGHSGTLDPKVTGCLIVCIDRATRLVKSQQGAGKEYVCVIRLHDKIPGGEAQFARALETLTGALFQRPPLISAVKRQLRIRTIHESKLYEFDNDRHLGVFWVSCEAGTYIRTLCVHLGL

Dictyostelium discoideum KTGHSGTLDPKVTGCLIVCIERATRLVKSQQGAGKEYIGIVRLHGAIEG-TTELSKAVDTLTGALFQRPPQKSAVKKRLRVRTIHNSKLLEFDPERNLGLIWVDCEAGTYIRTLCVHIGL

Arabidopsis thaliana KTGHSGTLDPKVTGNLIVCIDRATRLVKSQQGAGKEYVCVARLHSAVP-DVAKVARALESLTGAVFQRPPLISAVKRQLRIRTIYESKLLEYDADRHLVVFWVSCEAGTYIRTMCVHLGL

Chlamydomonas reinhardtii KTGHSGTLDPKVTGNLIVCIDRATRLVKAQQAAGKEYVCIARFHGKVEGGTAAVNKALETLTGALFQRPPLISAVKRQLRVRTIYQAKLYEYDEERHLAVFWISCEAGTYVRTLCVHLGL

Cryptosporidium parvum KTGHSGTLDPKVTGCLLVCLNRATRLVKSQQSAGKEYVCIVRFHSSVGG-KSAVSRCLETLTGALFQRPPVISAVKRQLRVRSIYDSKLLDYDEKRNLAVFWAKCEAGTYIRTMCVHMGL

Euglena gracilis KTGHAGTLDPKVTGSLLICIERATRLVKSQQNAGKEYVGVLRLHDALKD-EAQLLKALEDLTGAVFQRPPVVAAVKRTLRIRTIYESKLLDFDNDRHLAVFWISCEAGTYVRTLCVHLGF

Methanocaldococcus jannaschii KAGHGGTLDPKVTGVLPVALERATKTIPMWHIPPKEYVCLMHLHRDASE--EDILRVFKEFTGRIYQRPPLKAAVKRRLRIRKIHELELLDKDGKD--VLFRVKCQSGTYIRKLCEDIGE

250 260 270 280 290 300 310 320 330 340 350 360

....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|

                                                               |---------------------------------------PUA domain-------

Homo sapiens LLGVGGQMQELRRVRSGVMSEK--DHMVTMHDVLDAQWLYDNHKDESYLRRVVYPLEKLLTSHKRLVMKDSAVNAICYGAKIMLPGVLRY---------EDGIEVNQEIVVITTKGEAIC

Rattus norvegicus LLGVGGQMQELRRVRSGVVGER--DHMVTMHDVLDAQYLYDHHRDESYLRRVVFPLEKLLTSHKRLVMKDSAVNAICYGAKIMLPGLLRY---------EDGIEVNQEVVVITTKGEAVC

Mus musculus LLGVGGQMQELRRVRSGVMSEK--DHMVTMHDVLDAQWLYDNHKDESYLRRVVYPLEKLLTSHKRLVMKDSAVNAICYGAKIMLPGLLRY---------EDGIEVNQEIVVITTKGEAIC

Bos taurus LLGVGGQMQELRRVRSGVMSEK--DHMVTMHDVLDAQWLYDNHKDESYLRRVVYPLEKLLTSHKRLVMKDSAVNAICYGAKIMLPGVLRY---------EDGIEVNQEIVVITTKGEAIC

Gallus gallus LLGVGGQMQELRRVRSGILGEM--DNMVTMHDVLDAQWQYDNNKDDSYLRRVILPLEKLLTSHKRLVMKDSAVNAICYGAKIMLPGVLRY---------EDGIELKQEIVVITTKGEAIC

Danio rerio LLGVGGQMQELRRVRSGVLGEK--DNLVTMHDVLDAQWQFDHNKDETYLRRVIFPLEKLLISHKRIVMKDSAVNAICYGAKIMLPGVLRY---------EDGIEVNQDIVVITTKGEAIC

Drosophila melanogaster VLGVGGQMLELRRVRSGIQSER--DGMVTMHDVLDAMWLYENHKDESMLRRVIKPLEGLLVNHKRIIMKDSSVNAVCYGAKITLPGVLRY---------EDGIEIDQEIVICTTKGEAIC

Anopheles gambiae VLGVGGQMLELRRVRSGIQSEK--DGMVTMHDVLDAQYLYENHKDESMLRRVIKPLEGLLVGHKRIIMKDSSVNAVCYGAKILLPGVLRY---------EDGIEIDQEIVIVTTKGEAIA

Aedes aegypti VTGVGGQMLELRRVRSGIQSEK--DGMVTMHDVLDAQYLYDNHKDETMLRRVIKPLEGLLVGHKRIIMKDSSVNAVCYGAKIMLPGVLRY---------EDGIEMDQEIVIVTTKGEAVA

Culex pipiens quinquefasciatus VTGVGGQMLELRRVRSGIQSEK--DGMVTMHDVLDAQYLYENHKDESMLRRVIKPLEGLLIGHKRIIMKDSSVNAVCYGAKIMIPGVLRY---------EDGIEVDQEIVIVTTKGEAIA

Caenorhabditis elegans ILGCGGQMQELRRNRSGICDEN--ENMVTMHDVLDAQYLLDTQKDESYMRHIVRPLEALLTQHKRVVVKDSCINAICYGAKILIPGILRY---------DDDIEVGKEIVIMSTKGEAIC

Schizosaccharomyces pombe LLGVGGHMQELRRVRSGCLSEN--DDIVTMHDVLDAQWIYDNTRDESYLRRVIRPLESLLVGYKRIVVKDSAVNAICYGAKLMIPGLLRY---------EAGIEVNEEIVLITTKGEAIA

Saccharomyces cerevisiae LLGVGGHMQELRRVRSGALSEN--DNMVTLHDVMDAQWVYDNTRDESYLRSIIQPLETLLVGYKRIVVKDSAVNAVCYGAKLMIPGLLRY---------EEGIELYDEIVLITTKGEAIA

Kluyveromyces lactis LLGVGGHMQELRRVRSGALSEN--DNLVTLHDVMDAQWVYDNTRDESYLRKIIQPLETLLVGYKRIVVKDSAVNAVCYGAKLMIPGLLRY---------EEGIELYDEVVLITTKGEAIA

Ashbya gossypii LLGVGGHMQELRRVRSGALDEN--DNMVTLHDVLDAQWVYDNTRDESYLRKIIQPLETLLVGYKRIVVKDSAVNAVCYGAKLMIPGLLRY---------EEGIELYDEVVLITTKGEAIA

Lodderomyces elongisporus LLGVGGHMQELRRVRSGAMSES--DNLVTLHDVLDAQYVYDNTRDESYLRKIIQPLETLLVGYKRLVVKDSAVNSVCYGAKLMLPGLLRY---------ESGIELYDEVVLITTKGEAIA

Pichia stipitis LLGVGGHMQELRRVRSGALGEN--DNLVTLHDVLDAQYVYDNTRDESYLRKIIQPLETLLVGYKRIVVKDSAVNSVCYGAKLMIPGLLRY---------EEGIELYDEVVLMTTKGEAIA

Pichia guilliermondii LLGVGGHMQELRRVRSGALAEN--DNLVTLHDVLDAQYVYDNTRDESYLRKIIQPLETLLVGYKRIVVKDSAVNSVCYGAKLMIPGLLRY---------EEGIELYDEVVLITTKGEAIA

Zygosaccharomyces rouxii LLGVGGHMQELRRVRSGALSEN--DNLVTLHDILDAQWVYDNTRDESYLRRVIQPLETLLVGYKRIVVKDSAVNAVCYGAKLMIPGLLRY---------EDGIELYDEVVLITTKGEAIA

Aspergillus fumigatus LLGVGAHMQELRRVRSGAMDEN--NGLVTLHDVLDAQWMYDNQRDESYLRRVIQPLESLLTTYKRIVVKDSAVNAVCYGAKLMIPGLLRF---------EAGIEVNEEVVLMTTKGEAIA

Aspergillus clavatus LLGVGAHMQELRRVRSGAMDEN--NGLVTLHDVLDAQWMYDNQRDESYLRRVISPLESLLTTYKRIVVKDSAVNAVCYGAKLMIPGLLRF---------EAGIDVNEEVVLMTTKGEAIA

Aspergillus nidulans LLGVGAHMQELRRVRSGAMSEN--EGMVTLHDVLDAQWLYDNQRDESYLRKVIKPLESLLTTYKRIVVKDSAVNAVCYGAKLMIPGLLRFGQKSLTWGLEAGIELGEEVVLMTTKGEAIA

Chaetomium globosum LLGVGAHMQELRRVRSGVMSED-NGSLVTLHDVLDAQWQYDNGGDESYLRKVIQPLETLLCTYKRLVVKDSAVNAVCYGAKLMLPGLLRY---------DAGIDAHEEVVLMTTKGEAIA

Magnaporthe grisea MLGVGAHMQELRRVRSGAMDET--KDLHTLHDVLDAQWLMDNTRDESYLRKVIQPLETLLTTYKRLVVKDSAVNAVCYGAKLMLPGLLRY---------EADIELHEEVVLMTTKGEAIA

Neurospora crassa LLGVGAHMQELRRVRSGIMSED-NGSMVTLHDVLDAQWQFDNGGDETALRKVIQPLETLLTTYKRIVVKDSAVNAVCYGAKLMLPGLLRF---------EKGIETHEEVVLMTTKGEAIA

Botryotinia fuckeliana LLGVGAHMQELRRVRSGAMDET--KDMVTLHDVLDAQWMQDNARDEAYLRKVIQPLETLLTTYKRIVVKDSAVNAVCYGAKLMIPGLLRY---------EADIEVHEEVVLMTTKGEAIA

Pyrenophora tritici-repentis LLGVGAHMQELRRVRSGAMDET--KDLVTLHDVLDAQWLHDNNRDEAYLRKVIAPLESLLMSYKRIVVKDSSVNAICYGAKLMLPGLLRF---------EKGIEIHEEVVLMTTKGEAIA

Neosartorya fischeri LLGVGAHMQELRRVRSGAMDEN--NGLVTLHDVLDAQWMYDNQRDESYLRRVIRPLESLLTTYKRIVVKDSAVNAVCYGAKLMIPGLLRF---------EAGIDVNEEVVLMTTKGEAIA

Coccidioides immitis LLGVGAHMQELRRVRSGAMDEQ--DGMVTLHDVLDAQWMMDNNRDESYLRRVVRPLETLLTTYKRVVVKDSAVNAVCYGAKLMIPGLLRF---------EAGIEVHEEVVLMTTKGEAIA

Dictyostelium discoideum LMGVGGHMQELRRVRSGIMSEK--KGQVTMHDVKDAQYEYDNSKDESYLRRVIQPLEAILTNYKRIVVKDSAINAICYGAKLMIPGLLRY---------EQDIESNEEVVLITTKGEAIA

Arabidopsis thaliana LLGVGGHMQELRRVRSGILGEN--NNMVTMHDVMDAQFVYDNSRDESYLRRVIMPLEMILTSYKRLVVKDSAVNAICYGAKLMIPGLLRF---------ENDIDVGTEVVLMTTKGEAIA

Chlamydomonas reinhardtii LLGVGGHMQELRRVRSGIMGEAPSNNMVTMHDVLDAQWLYDNFKDESYLRRAVMPLEVLLTSFKRLVVKDSAVNAICYGAKLMIPGLLRF---------ESGVEVDDEVVLMTTKGEAVA

Cryptosporidium parvum LLGVGAHMQELRRVRSGCLSEN--DNMVTMHDILDAQYMLDNYRDESYLRRIISPLELLLTDLPRIVVKDSCVNAICYGAKLMIPGVLRF---------DNGIDVGTEVVMMTTKGEAIA

Euglena gracilis VLGTAGHMAELRRTRTGHLGEN--DGMVTMHDVLDAMHLYDQEGDESYLRRTVMPCELLLTAYKRVVVKDTAVNAICYGAKLMIPGLLRF---------ENGIEVNEVIVLMTTKGEAIA

Methanocaldococcus jannaschii ALGTSAHMQELRRTKSGCFEEK---DAVYLQDLLDAYVFWKEDGDEEELRRVIKPMEYGLRHLKKVVVKDSAVDAICHGADVYVRGIAKL---------SKGIGKGETVLVETLKGEAVA

370 380 390 400 410 420 430 440 450 460 470 480

....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|

-------------------------|                                                                                              

Homo sapiens MAIALMTTAVISTCDHGIVAKIKRVIMERDTYPRKWGLGPKASQKKLMIKQGLLDKHGKPTDSTPATWKQEY-------VDYSESAKKEVVAEVVKAPQVVAEAAKTA------------

Rattus norvegicus VAIALMTTAVISTCDHGVVAKIKRVIMERDTYPRKWGLGPKASQKKQLIKQGLLDKHGRPTDGTPASWTRDY-------VDYSDSSKKATAAEATPGPGVTADAASIV------------

Mus musculus MAIALMTTAVISTCDHGIVAKIKRVIMERDTYPRKWGLGPKASQKKMMIKQGLLDKHGKPTDNTPATWKQDY-------IDYSDSGKNTLVTEAVQAPQLAAEAVNVI------------

Bos taurus MAIALMTTAVISTCDHGIVAKIKRVIMERDTYPRKWGLGPKASQKKLMIKQGLLDKHGKPTDSTPATWMQEY-------VDYSNSAKKDVPPKAVKATPVVAEVVKTP------------

Gallus gallus LAIALMTTAVISTCDHGVVAKIKRVIMERDTYPRKWGLGPKASQKKMMIQKGLLDKHGKPNECTPDSWKKEY-------VDYRESSKK----EAAKVPQAVSEVERAP------------

Danio rerio TAVALMTTAVISTCDHGVVAKIKRVIMERDTYPRKWGLGPKASQKKMMIQKGLLDKHGKPNNSTPSDWKEGY-------VDYSTTKVK----KGG---------EASA------------

Drosophila melanogaster LAIALMTTATMASCDHGVVAKIKRVIMERDTYPRKWGLGPKASAKKALIAAGKLDKFGRPNENTPKEWLTGY-------VDYNAKKPAAQEVSPTNGSSEP-------S-----------

Anopheles gambiae LGIALMTTATMAGCDHGVVAKIKRVIMERDTYPRKWGLGPKASMKKQLIASGKLDKYGKPNENTPKDWLNSY-------SDFNR---SAKQANGNGTSADD---EFAGG-----------

Aedes aegypti LAIALMTTATMASCDHGVCAKIKRVIMERDTYPRKWGLGPKASMKKQLIASGKLDKYGKPNDKTPKEWLTGY-------VDFNKPAAPGKDTNGNGAAAAEGEDEFAGG-----------

Culex pipiens quinquefasciatus LGVALMTTATMASCDHGVCAKIKRVIMERDLYPRKWGLGPKASMKKQLIASGKLDKFGKPNDKTPKEWLTGY-------TDYNK----AKPSNGNQVAAEE-DDEVVGG-----------

Caenorhabditis elegans IAIAQMNTSTIASVDHGVVAKSKRVIMERDVYGRKWGLGPVASKKKQMVKDGLLDKFGKPNDTTPKSWAKEY-------VQTST------------------------------------

Schizosaccharomyces pombe VGIAQMSTVELSTCDHGVVAKVKRCIMERDVYPRRWGLGPQSMKKKTLKKEGKLDKYGRPNENTPADWSKSY-------IDYS--DPNAEVAKPAPVVAPAAPTVEAEVNGVEDS-----

Saccharomyces cerevisiae VAIAQMSTVDLASCDHGVVASVKRCIMERDLYPRRWGLGPVAQKKKQMKADGKLDKYGRVNENTPEQWKKEY-------VPLDNAEQSTSSSQETKETEEEPKKAKEDS-----------

Kluyveromyces lactis VAIAQMSTVDLATCDHGVVAKVKRCIMERDLYPRRWGLGPIAQKKKQMKADGKLDKYGRANENTPETWKKTY-------VSLENAEPTTAPASKSEE-KPLIKEVE--------------

Ashbya gossypii VAIAQMSTVDLATCDHGVVAKVKRCIMERDLYPRRWGLGPVAQKKKQLKADGKLDKYGRVNENTPEDWKKTY-------VSHE-PSADAAAEVAPVKSEPLIEEVS--------------

Lodderomyces elongisporus IGIAQMTTVDLQSCDHGVVAKVKRCIMERDTYPRRWGLGPVAQKKKQLKADGKLDKYGRVNDETPETWKKEY-------KDLD--EAPAPAIPESKLVAPAPQ-LP--------------

Pichia stipitis IGIAQMTTVDLQGCDHGVVAKVKRCIMERDTYPRRWGLGPVAQKKKQMKADGKLDKFGRANENTPETWKQEY-------KDHD--EEPAPAVPASKLVAPEVQ-LP--------------

Pichia guilliermondii IGIAQMTTVDLQSCDHGIVAKVKRCIMERDTYPRRWGLGPVAQKKKQLKADGKLDKYGRTNENTPESWKAEY-------KDHD--ETPAPPVPEAKLAAPEIQ-LP--------------

Zygosaccharomyces rouxii VAIAQMSTVDLATCDHGVVAKVKRCIMERDLYPRRWGLGPVAQKKKQMKVDGKLDKYGRVNESTPEDWKKSY-------VPLDNAEAPAARESAPKESAPLVQEVD--------------

Aspergillus fumigatus IGIAQMSTVELSTCDHGVVAKVKRCIMERDLYPRRWGLGPVALEKKKLKSAGKLDKYGRANEDTPAKWKTEY-------KDYSAPEEASA-HAASESTAKEDVAAALTT--------EQD

Aspergillus clavatus IGIAQMSTVELSTCDHGVVAKVKRCIMERDLYPRRWGMGPVALEKKKLKSAGKLDKYGRTNEATPAKWKTEY-------KDYNAPEDAAA-QPATETNGTEEAAAAPAA--------EQA

Aspergillus nidulans IGIAQMSTVELSTCDHGVVAKVKRCIMERDLYPRRWGLGPVALEKKKLKSSGKLDKYGRANEATPAKWKSEY-------KDYSAPDGDSS-QQAVDVVAKEEASP------------KEE

Chaetomium globosum IGIAQMSTVEMSTCDHGVVAKVKRCIMERDLYPRRWGLGPVAMEKKKLKADGKLDKFGRTNEATPAKWQQGY-------TDYGAA---DAAAAAAASAAAAPLEA--------------A

Magnaporthe grisea IGIAQMSTVEISSCDHGVVAKVKRCIMERDLYPRRWGLGPVAGEKKKLKADGKLDKFGRPNEATPSRWTQNY-------KDYSAPAPDAAAPAAD--EAAAATESP-------------A

Neurospora crassa IGIAQMNGVEMLSCDHGVVAKVKRCIMERDLYPRRWGLGPVALEKKKLKSDGKLDKFGRPNAETPAKWTEGY-------KDFSAAAESEAAAAAAPAAEAAKVEI--------------A

Botryotinia fuckeliana LGIAQMSTVELSSCDHGVVAKVKRCIMERDLYPRRWGMGPVAIEKKKMKTEGKLDKYGRPNEATPAKWNNEY-------KDFNAPPEDGAIAIHPKPEVVASAVAP-------------A

Pyrenophora tritici-repentis LAYAQMSAVEMSSCDHGVVAKIKRVIMERDLYPRRWGMGPVATEKKKMKEAGTLDKFGRPNDKTPAKWNTEY-------KDFNRNDVDGASAPVAQTTSTAEVLAAPAI--------PAT

Neosartorya fischeri IGIAQMSTVELSTCDHGVVAKVKRCIMERDLYPRRWGLGPVALEKKKLKSAGKLDKYGRANEDTPAKWKTEY-------KDYSAPEEASA-QAAPGSTAKEDVAAALTT--------EQD

Coccidioides immitis LGIAQMSTVEMSTCDHGVVAKVKRCIMERDLYPRRWGMGPVALEKKKMKADGKLDKFGRPNASTPAKWQAEY-------KDYSASADAAAPQPATEATPATPAQAPAAS--------PEE

Dictyostelium discoideum IGIAQMTTASMATCDHGVVATIKRVIMDRDVYPVRWGLGPKAKVKKEMIKDGKLDKFGKPNEKTPSDWTNTY-------TYYTGQVATTTTTSPVESMNVDTPKKATASVA--------V

Arabidopsis thaliana VGIAEMTTSVMATCDHGVVAKIKRVVMDRDTYPRKWGLGPRASMKKKLIADGKLDKHGKPNEKTPVEWSRNV-------VLPTGGDAIIAGAAAAPEEIKADAENGEAGEARKRKHDDSS

Chlamydomonas reinhardtii VGIAQMTTGVMATVDHGCVAKIKRVIMERDTYPRRWGLGPYAQIKKKLITEGKLDKHGRPNENTPKEYLR---------SLP---DFVANGGASAAAAAPAAAT----------------

Cryptosporidium parvum IGVAQMTTAVIASVDHGVVAIIKRVIMERDTYDMRWGHGPRASEKKKLILGGKLDKHGKPNEQTPESWLKYEGYIPKLTGDHDDQNDEGKDITGSGLESESNSNIESSS-SKKRKEKKET

Euglena gracilis LGIALMTTADMGTCDHGIVAKIKRVVMDRDTYPRRWGLGPVALKKKEMIERGHLDKHGRRTEATPNSWW--Y-------VDYSG------CGSNEEAMNREGNK--SLGPP--------P

Methanocaldococcus jannaschii VGKALMNTKEILNADKGVAVDVERVYMDRGTYPRMWKRKK--------------------------------------------------------------------------------

490 500 510 520 530 540 550 560 570 580 590 600

....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|

                                |NL|                                                                   

Homo sapiens --------------------------------KRKRESESESDETPPAAPQLI-------KKEKKKSKKDKK------AKAGLESGAEPGDGDSD--TTKKKKKKKKAKEVELVSE----

Rattus norvegicus --------------------------------KRKRDSDSDADEATPTTTPRV-------KKEKKK-KKEK----------ADGGEEAAEDGDGD--ATRKKKKKK-ARAAEELSG----

Mus musculus --------------------------------KRKRDSESES-DETPTVPQLK-------EKKKKKDKKPK---------TVLESGGETGDGDND--TTKKKKKKK-VKVVEEMSE----

Bos taurus --------------------------------KRKRESESE--DTSPAAPQVV-------KKEKKKKKKEK-------AKAAAEK---PGAGDSD--STKKKKKKK--IKAEMVSE----

Gallus gallus --------------------------------KRKRESESENEAVSPPPSPATPPPEELSKKEKKKKKKEKK------AKEAAESGEEQ-VEVISESSAKKKKKKKKQKEVEESSE----

Danio rerio --------------------------------KRKRD-ESGSEGEAAAAAQDTSKTEEESKKEKKKKKKEKK------QKLAEEAAAEAPAEEETEVTESAKKKKKKKK-----------

Drosophila melanogaster --------------------------------KRKLST-SSVEETAAAAVSEET-PSKDKKKKKKKHKGDEE----------APEAAEEEA-EPVEKEKKKKKKKDKDRDRDEAQE----

Anopheles gambiae --------------------------------KRKLSIGENVES-----AATIG-DGEEKKKKKKKQKKDAEDGDGEAVGAEGQPMDQAEGDEEMKKEKKKKKKKDKNVSVDEVAE----

Aedes aegypti --------------------------------KRKLSVGSAADQSMETSAMEVD-AGEKKKKKKKKHKKDDE--APEAP--ETPEAVEVEATEEVSETK--KKKKKKDKKQEEEAE----

Culex pipiens quinquefasciatus --------------------------------KRKLSIGSAADNSMDTSAIDAG-EGKEKKKKKKKQKQDEE--APEEAAAEATEPAEADATEETSESKKEKKKKKKDKKQQEEAE----

Caenorhabditis elegans --------------------------------KKEVKKEETPDEE---------EEEAPKKKSKKSKKQESSDSDSD-------------------------------------------

Schizosaccharomyces pombe --------------------------------KKRKSVESSEKDED----EAAKKEEKRRKKEAKKEKKEKKEKK--------------EKKE-------KKKKSE--------------

Saccharomyces cerevisiae -------LIKEVETEKEEVKED----------------DSKKEKKEKKDKKEKKEKKEKKDKKEKKEKKEKKRKS--------------EDGD--SEE-KKSKKSKK-------------

Kluyveromyces lactis ----KKEVEQKEESKEESKTPE-----------------------EKKDKKEKKEKKDKKEKKEKKEKKEKKRKA--------------DDDE--SSE-KKKKKSKK-------------

Ashbya gossypii ----KKDAKTEVEVPAEKKQDD-------------EEKKEKKEKKEKSEKKEKKEKKEKSEKSEKKEKKEKKRKA--------------DDEN--ASEEKKKKKSKK-------------

Lodderomyces elongisporus ----KKTLIEEVEVST------------------------VEVDGDKKEKKEKKDKKDKKDKKDKKDKKEKKRKA--------------EDDEGESSKSEKKKKSKKD------------

Pichia stipitis ----KKTLIEEVASEEASEA--------------------EEKKEDKKEKKEKKEKKEKKDKKEKKDKKDKKRKA--------------EDGE---EKAEKKKKAKK-------------

Pichia guilliermondii ----KKLIEEVETKSSLDDDM------------------EEESKKEKKEKKEKKEKKEKKEKKEKKEKKEKKRKS--------------EDGE---TESGKKKKSKKE------------

Zygosaccharomyces rouxii ----KKDEEEKKEEKKEEKKEEKEEKKEEAEESDDEEKSDKKSKKEKKDKKEKKDKKEKKEKKEKKEKKEKKRKA--------------DDDE--DGE-SKKKKSKK-------------

Aspergillus fumigatus EAPSSPQSKMDVDETKEE-------------KKRKRHEG----------ETAEERAERKRKKKEKKEKKERRKSKQEKE---------DSDDSD--------------------------

Aspergillus clavatus EAPSSPQSKMDVDEDKDE-------------KKRKRHEG----------ETPEERAERKRKKKEKKEKKERRKSKQEKD---------DSDDSD--------------------------

Aspergillus nidulans PSLEANESKMDIDDAQDDE----------DKKKRKRHEG----------ETPEERAERKRKKKEKKEKKERRKSKQEKD---------DSDDSD--------------------------

Chaetomium globosum SPQKQIEAAPATEEKADE-----------ESKKRKKHEG----------ETAEEKAERKRLKKEKKEKKEKKKGKEAA----------DESD----------------------------

Magnaporthe grisea KALPAPEAEPEAEADDGK-------------KRKSKHEG----------ETAEEKAERKRRKKEKKEKRASKAAAE------------EDSD----------------------------

Neurospora crassa ERPKPEEKAEKSEEAEEAG----------DSKKRKKHDG----------ETAEEKAERKKQKKEKKEKKEKKEKKSKKGG--------DESD----------------------------

Botryotinia fuckeliana LAPVDGNAAEDDEEKSD--------------KKRKRHDG----------ETAEEKAERKRKKKEKKAKRESKKS----AG--------GSSDSE--------------------------

Pyrenophora tritici-repentis GLTSTEEAEAEAEAGAADA--------DSKKSKKRKSRG------DGEEEADEDKAERKKKKKEEKAAKKAAKKDKRKSKG------GDDSDSD--------------------------

Neosartorya fischeri EAPSSPQSKMDVDETKEE-------------KKRKRHEG----------ETAEERAERKRKKKEKKEKKERRKS-KQEKE--------DSGDSD--------------------------

Coccidioides immitis ASSPAP------EADKED------------AKKRKRHDG----------ETPEEREERKRRKKEKKEEKEKRKQEKKEKKERRKSKQADSSDSE--------------------------

Dictyostelium discoideum KAEVESSEDEKPVPKKEKKD-----------KKEKKKDSS--------DDESEEEKSSKKDKKEKKEKKEKKEKKSSKDDSDDESSKKEKKDKKRKKSSDKDSDSEKESSDKKDKKDKKE

Arabidopsis thaliana DSPAPVTTKKSKTKEVEGEEAEEK---VKSSKKKKKKDK-----------EEEKEEEAGSEKKEKKKKKDKKEEVIEEVASPKSEKKKKKKSKDTEAAVDAEDESAAEKSEKKKKKKDKK

Chlamydomonas reinhardtii -------------------------------PVEIKEEG----------------------KKEKKVRRWGKS-----------------------------------------------

Cryptosporidium parvum NDEESSSSFSSKN----------KKEKNKRKSTEPLSENGSSSSSSDSSSSNSSSDDEKSKSKGKKSSKDKSKKKKKHSR--------ISED----------------------------

Euglena gracilis KVRIEELAYAEAAKKRER------------------------------EAAGEDEKDAKKAKKEKKEKKEKKEKK-------------EKAEKTA-------------------------

Methanocaldococcus jannaschii ------------------------------------------------------------------------------------------------------------------------

610

....|....|.

Homo sapiens -----------

Rattus norvegicus -----------

Mus musculus -----------

Bos taurus -----------

Gallus gallus -----------

Danio rerio -----------

Drosophila melanogaster -----------

Anopheles gambiae -----------

Aedes aegypti -----------

Culex pipiens quinquefasciatus -----------

Caenorhabditis elegans -----------

Schizosaccharomyces pombe -----------

Saccharomyces cerevisiae -----------

Kluyveromyces lactis -----------

Ashbya gossypii -----------

Lodderomyces elongisporus -----------

Pichia stipitis -----------

Pichia guilliermondii -----------

Zygosaccharomyces rouxii -----------

Aspergillus fumigatus -----------

Aspergillus clavatus -----------

Aspergillus nidulans -----------

Chaetomium globosum -----------

Magnaporthe grisea -----------

Neurospora crassa -----------

Botryotinia fuckeliana -----------

Pyrenophora tritici-repentis -----------

Neosartorya fischeri -----------

Coccidioides immitis -----------

Dictyostelium discoideum KKKSKN-----

Arabidopsis thaliana KKNKDSEDDEE

Chlamydomonas reinhardtii -----------

Cryptosporidium parvum -----------

Euglena gracilis -----------

Methanocaldococcus jannaschii -----------