10 20
30 40
50 60 70 80 90 100 110 120
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
Schizosaccharomyces pombe ---------------------------------------MTEHHTPKSRILRFLENQYVYLCTLNDYVQLVLRGSPASSYSNICER-------------LRSDVQTSFSIFLHSTVVGFD
Saccharomyces cerevisiae
------------------------------------------------------------MKILFEFIQDKLDIDLQTNSTYKE--------------NLKCGHFNGLDEILTTCFALPN
Saccharomyces paradoxus ------------------------------------------------------------MRSLYEFTQDKLNIDLETCIIYQE--------------NFKCGQFNGLDEILTTCFILQN
Saccharomyces mikatae ------------------------------------------------------------MKSLYDFIQDKLEIELETNITYEE--------------NIESGQFNGLDEILTTCFILQN
Saccharomyces kudriavzevii ------------------------------------------------------------MKSLYEFIQDKLGTDFESNALNQD--------------NFECGQFNGLDELLTTCFILPN
Saccharomyces bayanus ------------------------------------------------------------MISLSEFIHDKLNTDLETNALHHD--------------VLKYGQFNGLNELLTTCFILPN
Kluyveromyces lactis ------------------------------------------------------------MKILVEYLES---LECYETLRKSD--------------ILINADSNGVSNLLLSCFAQVN
Candida albicans 1 ------------------------------------------------------------MTVKVNEKKTLLQYVLDNTSNDVP--------------LLPS-----LKEYMETVLVYKS
Candida albicans 2 ------------------------------------------------------------MTVKVNEKKTLLQYVLDNTSNEVP--------------LLPS-----LKEYMETVLVYQS
Ashbya gossypii MSFKEKRK--------------------LEATDASSDRKRVRTSRDTLVKRDLLDRCYDKVTTLREYILLKLPPRSRLRRKKVASVGE---------GDDVGELESNLSRLLDTSLVCFD
Aspergillus fumigatus MGQKRKRPVKDGCAAD-----NSALRRIISASSGSRNHGASARNEKDGHSHPVISLYYRQVVTLRQYLLQQIPVTSKSRRRRIASIRSDPLTQREGDDSPGDRQVQDLASVLDTTLVGVL
Aspergillus clavatus MGKKRKRPSKDSGAGE-----DLAQKRLAGGPNGVRSHHATDAYEGSGHSHPVISLYYHRVVTLRQYLLQQIPVSSKSRRRRITSVQGATSTEKQKNRGVRGYHAQGLADILDTTLVGVL
Aspergillus nidulans MGKKRKRPVKEPVKDDPICRAKSSPQQPAGNTARSLSTNRNQAADAGKICHPVISLYYRHVVTLRQYILQRIPRSSRARRRRIAAVGG--HSAARDGLAAVPKNEKDLADLLDTTLVGIL
Neosartorya fischeri MGKKRKRPIKDGCTAD-----NSAPRRIISTSGGSRNHGASARKEKDGHSHPVISLYYRQVVTLRQYLLQQIPVTSKSRRRRIASIRSDPLTQSQGDDSSGERQVQDLASVLDTTLVGVL
Encephalitozoon cuniculi
----------------------------------------------------------MKIYKSRQMLELLKSFGFLSLRDFLAAYG-----------------VADLPDIVDRVYVRAS
130
140
150
160
170
180
190
200
210
220
230
240
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
Schizosaccharomyces pombe SKP-------------------------DEGVQFSSPKCSQSELIANVVKQMFDESFE---RRRNLLMKGFSMNHEDFR----AMHVNGVQNDLVSTFPNYLISILESKNWQLLLEIIG-
Saccharomyces cerevisiae
SR--------------------------KIALPCLPGDLSHKAVIDHCIIYLLTGEL-----YNNVLTFGYKIARNED-----------VNNSLFCHSANVNVTLLKGAAWKMFHSLVG-
Saccharomyces paradoxus SR--------------------------KVALPYLPGDLSHKAVIDHCIIYLLTGEF-----YNNVLTFGYKIARNED-----------VDNSLFCHSANVNVTLLKGVPWEMFHSLIG-
Saccharomyces mikatae SR--------------------------KIALPSVSGDLNHKAIIDHCIVYLLTGEF-----FNNVLTFGYKIAKNED-----------VDNSLFCHSTNVSVTLLKGTAWKMLHNLIG-
Saccharomyces kudriavzevii SR--------------------------KIALPSISGDLNHKSVVDQCITYLLSGDF-----FNNVLTFGYRIARNEN-----------VNNSLFCHSTNVSVTLLKSTTWRMFHDLIG-
Saccharomyces bayanus SR--------------------------KIALPSIAGDLNHKSVIDQCIIHLLTGDF-----FNNVLTFGYRIARNEN-----------VDNSLFCHSTNVSATLLKSSTWELLHGLIG-
Kluyveromyces lactis SKH---------------------CVPPEIEISSTFSSSYHCETIETCILYLLHKNF-----TNNILTYGYQLGKNSD-----------VMTKLFCRSSNISVSVLKCNSWRLFHQLVG-
Candida albicans 1 IKR---------------------------PLPAIRPQESFDEFMKELVTRLVMEK------SNNVIAYGYKTSAMESR----------SIFTTFHSSGNFILTHITSHNWSTIFSLLG-
Candida albicans 2 IKR---------------------------PLPAIRPQESFDEFMKELVTRLVMEK------SNNVIAYGYKTSAMESR----------SIFTTFHSSGNFILTHITSHNWSTIFSLLG-
Ashbya gossypii NDQQNVKDVDMRKKQLIEFTQKGDESYVSLSDGISGSVFSQDEIMDFVVWLLFQRE--PHARPKHILCDGFQKLAGQN------GQGTTNIPDLSIRHHNSHVKALRESPWPQLLALLG-
Aspergillus fumigatus NET-SPTVTQERRRDFAAYSQTQSQCRSELTSTDTGPPCSQSEIVDFVITSLFNRNGFSYHRPQHLLAHGYQRAGASERMTDNLAAPTSSIPGLVVRFPNRNVETLKHAPWTNVLALLG-
Aspergillus clavatus KES-SPTVTQERLRDFASYSQSQSQGRSEVPSTDVGPPCAQSEIVDFVISALFNRHAFSYQKPQHLLTHGFQRAGGPD------VKLASSIPGLARRYPNKNVQCLKQAPWTDVLGLLG-
Aspergillus nidulans KEL-PPTRSEERRRDFIAFTQAQQTTQT---GTDSGP------IVDFAISSIFNRP--SHGKLENVLSHGYRQG---------GGRLPCSIPNVAAQFPNKNVQMLKQSPWTEVLALLG-
Neosartorya fischeri NET-SPAVTQERRREFAAYSQTQSQCRSELTSTDTGPPCPQSEIVDFVITSLFNRNGFSYHRPQHLLAHGYQRVGAPERMTDNHAAPTSSIPGLVVRFPNRNVETLKHEPWTNVLALLG-
Encephalitozoon cuniculi
RTP---------------------------VVSSFKSTQKDVELISKAVRTLLIKNR------DNHLCAGYKWWSPGLPG---------ASTKIICYFPNSSSRILRSHEWSWLMRSIGD
250
260
270
280
290
300
310
320
330
340
350
360
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
Schizosaccharomyces pombe SDA---MHYLLSKGS--IFEALPNDNYLQISGIPLFKNNVFEET--------------------------------------------------------VSKKRKRTIETSITQNKSAR
Saccharomyces cerevisiae
TYA---FVDLLINY---TVIQFNGQFFTQIVGNRCNEPHLPPK-----------------------------------------------------------------------------
Saccharomyces paradoxus TNS---FVDLLINY---TVVQFNGQFFAQIVGNRCNEPHLPPK-----------------------------------------------------------------------------
Saccharomyces mikatae TNA---FVDMLINH---TVIQFNGHFFTQIIGNRCNEPHLPPK-----------------------------------------------------------------------------
Saccharomyces kudriavzevii TYA---FVDILVNY---TVIQFNGQFFSQIVGNKCNEPHLPPK-----------------------------------------------------------------------------
Saccharomyces bayanus THA---FVDLLINY---TVVQFNGKFFTQIVGNRCNEPHLPPK-----------------------------------------------------------------------------
Kluyveromyces lactis TDN---FVNLLINY---SVYFNTGHYFRQIIGTPANAPQVPPA-----------------------------------------------------------------------------
Candida albicans 1 PKK---FLELLVN---------NKGFVSKVNGE--SVQIFGDV-----------------------------------------------------------------------------
Candida albicans 2 PKK---FLELLVN---------NKGFVSKVNGE--SVQIFGDV-----------------------------------------------------------------------------
Ashbya gossypii KAGEEIMIKLLVDASIYVAVEAGLNNYHQLSGVPLAELD---------------------------------------------LPGGNVSLNKGSAPSDSSNLDAGS------------
Aspergillus fumigatus GNGEEIMLRLLLDCGIFTAIDHRKGIYYQLSGLPLSSLEPVKR---QLPQEVSDLSGASIKTANNTGGFCSGPQHRKSVRQEVVHKPNSIVFFRRRMLYAKPVLNSKGDVHFGLTRLHVL
Aspergillus clavatus GNGEEIMLRLLFDCGIFMAIDHRKGIYYQISGLPLSSLEPIQN---QSPLEVPNANSKPSLAAGSN-----VARKNRSSDQGIAHKPNSVVFFRRRMLYAKPILNSRGEVHFGLSALHVL
Aspergillus nidulans SNGDEIMLKLLLDCGLFMAVDARKGVYCQISGQAISSLKPIDTSPEDCPAAFNGSSSPVKRHAVPWQGAAPRAHTKGPKENQQQLSPNAIIFCRQRMLYARPHLNANGGITFGLPN-HVL
Neosartorya fischeri GNGEEIMLRLLLDCGIFTAIDHRKGIYYQISGLPLSSLEPVKR---ELPQEVSDLSGASIHTANSTGGFCSGLKKRKSVRQEVVHKPNSIVFFRRRMLYAKPVLNSKGDVHFGLTRLHVL
Encephalitozoon cuniculi
ELAFFILTECLVIQMLNSGYVFLAGDFKSLSGMATER-----------------------------------------------------------------------------------
370
380
390
400
410
420
430
440
450
460
470
480
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
Schizosaccharomyces pombe KEVSWNSISISRFSIFYRSSYKKFKQDLYFNLHSICDRNTVHMWLQWIFPRQFGLINAFQVKQLHKVIP--------LVSQSTVVPKRLLKVYPLIEQTAKRLHRISLSKVYNHYCPYID
Saccharomyces cerevisiae
--WAQRSSSSSATAAQIKQLTEPVTNKQFLHKLNINSSSFFPYSKILPSSSSIKKLTDLREAIFP-------------TNLVKIPQRLKIRMNLTLQKLLKRHKRLNYVSILNSICPPLE
Saccharomyces paradoxus --WAQRTS--SGNTAQIKQLMGPVTNKPFLHRLKINSPSFFPYGKILPLSSSFKKLTDVRETIFP-------------INLVKIPQRLKVRINLTLQKLLKRHKRLNYVSVLNSICSPSE
Saccharomyces mikatae --WAQRSSPSSGSIAQIEPVMEPVTNKPFLHKLKLNCPFIFPYKTILSLSSSIEKLIELRENIFP-------------SYLVKIPQKLEVQINLTLQKLLIRHKRLSYVSILNGICPRLK
Saccharomyces kudriavzevii --WAQRASSSSERTVQMKQLTRPVTNKSFLHKLKTNSSTFFPYGRILSSSSSINRLSELREAIFP-------------SNLIKVPQRLKIGIDLTLRKMLTRHKGLNYVSILNNICPPLI
Saccharomyces bayanus --WVKKSSSPSDTTLLMKQLMEPITNKPFLHRLKINSPNFFPYGRILPSSSTLNKLTELRDAVFP-------------PNLVKIPQSLRIRVNITLQKLLTRHKRLNYVSILNNICPQLK
Kluyveromyces lactis --WLDRSYRRERNTKLTIDWNSALYHKIRPQDYKN----IIETDQGLLIAEIFPKISESSKTPRS-------------LNFALN------NLKPILYELIRAHERFSYRHIINNICPKSD
Candida albicans 1 --NSHRKAVVVSKYITKFNVLYNSYSRDFSRFEMIRPSIQTILQDILSFSGLNPGRSSKRYRGFK-------------SLLSRIIANDKKCRYDILYAKFIGTSKCNFANVVS-------
Candida albicans 2 --NSHRKAVVVSKYITKFNVLYNSYSRDFSRFEMIRPSIQTILQDILSFSGLNPGRSSKRYRGFK-------------SLLSRIIANDKKCRYDILYAKFIGTSKCNFANVVS-------
Ashbya gossypii ---------HGVIKIMMYMFPRQFGLHNVFTFQVDHTKTSQKFQDYTSREEEIAPIFQAKPGDAASRKP-------------KIPKRLRGDVEQLVKRLQILHSRCSYTELLSYYCSCVF
Aspergillus fumigatus NRYPRSDSLSETVHILKYIFPRQYGLHNVFTSVTDSRETVMPFKDYFSRETEIAQMDEQKRKRRQRIGPGFMNHFEKHKDLVEIPKRLRGKAVELVQQLQNRNRRCPYVKLLDYYCPADD
Aspergillus clavatus NRYPRSDSLSQTVHILKYIFPRQFGLHNVFTSVTDSRETVLPFKDYFSRETEIEEIEEKRRKQRRRIGPEFTNHYQDQKEFIDIPKRLRGKACELVQQLQNRNRRCPYTELLNYYCPPVD
Aspergillus nidulans SRFHSAKSLQQTVHVMKYVFPRQFGLHNVFTSHSSYYENPLS-KNYSSREEEIARKEGLEAARNQLRKSGFHAAIGERQESIKIPKRLRGKPLELIRQLQNRSRRCCYKKLLQYYCSEEL
Neosartorya fischeri NCYPRSDSLSETVHILKYIFPRQYGLHNVFTSVTDSRETVLPFKDYFSRETEIAQIDEQKRKRRQRIGPGFTNHFEKHNDLIEIPKRLRGKAVELVQQLQNRNRRCPYVKLLDYYCPIED
Encephalitozoon cuniculi
-------------------VSNAIGRNVLFYKETRRISFCSLSAFEYVFEAHNSEKYKVIQKDAR----------------------------DVLESIERRWKKISTHAIFKSFFKDEL
490
500
510
520
530
540
550
560
570
580
590
600
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
Schizosaccharomyces pombe T------------------------------------------------------------------HDDEKILSYSLKPNQVFAFLRSILVRVFPKLIWG----NQRIFEIILKDLETF
Saccharomyces cerevisiae
G-----------------------------------------------------------------TVLDLSHLSRQSPKERVLKFIIVILQKLLPQEMFG----SKKNKGKIIKNLNLL
Saccharomyces paradoxus E-----------------------------------------------------------------TVSNLSHLSRQSPKEQVLRFIIVILQKLLPQEMFG----SKKNKCKIIRNLNLL
Saccharomyces mikatae E-----------------------------------------------------------------TTSELPHLSRQSPKEEVLKFVIVILQKLLPQEMFG----SKKNKCKIIKNLNSL
Saccharomyces kudriavzevii E-----------------------------------------------------------------NGSELSHLGWQLPKEQVLRFLIVILEKLLPKEMFG----SKKNKCKIIRNLNSL
Saccharomyces bayanus E-----------------------------------------------------------------NASELPHLKSQLPQSQVLKFLIVILQKLLPQEMFG----SKRNKCKIIQNLNSL
Kluyveromyces lactis --------------------------------------------------------------------------TFYSSPKSVIKLLIVCVRKTFPLDLLG----SNSNYSVLSKAIAIL
Candida albicans 1 ---------------------------------------------------------------------------NKTEISQVIQFVLLVLGKLLPLDAWG----GVSNKKIIKDRVVDF
Candida albicans 2 ---------------------------------------------------------------------------NKTEISQVIQFVLLVLGKLLPLDAWG----GVSNKKIIKDRVVDF
Ashbya gossypii DRPSQIRKPKAKKIPMSTRKPKPSQHPFS--RSNQKCVSSQMHPEAGTSG-----------TQVQSLPEHDSLVELATPSFQVSSFCRAVLSKIIPDSFWGDKDAGKHNKSIAMRNVDHF
Aspergillus fumigatus VWPWKLSHTNSQEGSTGEEPRTPSDRLVTQPRGAEQHAARDVGPKESPKIRYSTHDTEDGTTKCLVPEPKQNLMDYATPSSSVSAFCRAVLRKLIPPQFYGIGQYQKSNQEIIFSHVDRF
Aspergillus clavatus TGPWKLGPTDSQTSHPSG--TAISESLVTQRRGPQGSFAHAS-PLSHLQETPSVATFKAEATVSKILKPKLCLIDYATPCSSVSAFCRAALRKLVPPRFYGTGCHQRSNQDIVFRHVDRF
Aspergillus nidulans SGPWILGQLSAESNSVLS--TSSSRPLVTQPSLAHQDMQRELRPTSYAKG---------FSKGSGATKPKENLTDHATPAASVSAFCRAVIRNLIPLEFFGVGEQGITHQKMILGHVDRF
Neosartorya fischeri VGPWKLGHTNSQEGSTGKGSCTASDQLVTQPRGAQQHAARDMGPKESLRTRNSIHDTEKGITKCVIPEPKQSLMDYATPSSSVSAFCRAVLRKLIPPQFYGIGQYQKSNQEIVFSHVDRF
Encephalitozoon cuniculi
AIE-----------------------------------------------------------------DKSSALECGVKQSKLVNFLFLISKKLFKDAFDL------HGFRILKSRLSLL
610
620
630
640
650
660
670
680
690
700
710
720
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
|-----------------------Motif
T-----------------| |--
Schizosaccharomyces pombe LKLSRYESFSLHYLMSNIKISEIEWLVLGKR------SNAKMCLSDFEKRKQIFAEFIYWLYNSFIIPILQSFFYITESSDLRN-RTVYFRKDIWKLLCRPFITSMKMEAFEKIN-ENNV
Saccharomyces cerevisiae
LSLPLNGYLPFDSLLKKLRLKDFRWLFISDI---------WFTKHNFENLNQLAICFISWLFRQLIPKIIQTFFYCTEISSTVT--IVYFRHDTWNKLITPFIVEYFKTYLVENN-VCRN
Saccharomyces paradoxus LSLPLNGYLPFDSLLKKLRLKDFRWLLVSDV---------SFTKQNFENLNQLVTCFISWLFRHLFPKIIQTFFYCTEISSTVT--IVYFRHDIWNKLVTPFLAGYFKKYLVENN-VCRN
Saccharomyces mikatae LSLPLNGYLPFDSLLKKLKFKDFRWLMVSTI---------SFTRQNFENLNQMVICFVSWLFRQLLPKVIQTFFYCTEISSTVK--IVYFRHDVWNGLVSPFLTGYFKRYLVENN-VCRN
Saccharomyces kudriavzevii LSLPLHGCLPFNSLMKNLRLKDFKWLLVSDS---------SFTKQNFENSNQLVIYFVSWLFRQLIPKIIQTFFYCTEISSTVA--IVFFRHDILDKLVAPFLREYFEKYLIENS-ICRN
Saccharomyces bayanus LGLPLNGHLLFGDLLKKLRLKDFQWLMVSDV---------SFTKQNFENSNLLMTFFVSWLFRHLVPKIIQTFFYCTEISSTVT--IIFFRHDTWNKLVTPFLAEYSEKYLTENI-VCRN
Kluyveromyces lactis VKKPLHSKILFDELCKGLRVKDVKWLETRRLPAGEQT--QKIPYYDVKNRQALLYKLFFWILSCYVPKLLSTFFYVTELSSTVD--IVYIRHDTWKTMSQPFLKSYFRRYLLENK-YCFQ
Candida albicans 1 LLLGANEKIHMDDLFRGIRLKDFKWLGRAHQ---------ISSKQDFELRTAFLKGYLWWLFEHLLKNILRSFWYITETSSIVSSELNYFPQYLWKELYESWVSKYAKNNLVKMP-SKIQ
Candida albicans 2 LLLGANEKIHMDDLFRGIRLKDFKWLGRAHQ---------ISSKQDFELRTAFLKGYLWWLFEHLLKNILRSFWYITETSSIVSLELNYFPQYLWKELYESWVSKYAKNNLVKMP-SKIQ
Ashbya gossypii IRLRRFETMSLHELTQDLKIAEISWL------QPHNTGTQKPSRTDTEKRLEIFHEFLYFVFDSLLIPLIRNNFYVTESNTHRY-RVFYFRHEVWRRIAEPAMADLKADMFEEVKLDDAL
Aspergillus fumigatus IRMRRFESLSLHEVCKGIKITSIPWLDYSRHQHQESLPGRKLSLSDLHKRTELLHEFIYYIFDSILIPLIRSNFYVTESQIYRN-QLFYFRHDVWRHLTLQPLATFKTTLFEEMKRADAE
Aspergillus clavatus VRMRRFESLSLHEVCKGIKITCMPWLGHPANQQDEGSSQPKMSLSDLRKRTELLHEFIYYIFESILVPLIRSNFYVTESQSHRN-RLFYFRHDVWRQLTQQPLAEFKTSLFEEMKRGVAE
Aspergillus nidulans IRMRRFESLSLHEVCEGIKPE----------SLRQAPSENNISASDLQKRRELFHEFLYYLFDSILLPLIRGSFYVTESQVHRY-RLFYFRHDVWRRLTAQPLAHLRASIFEELAPETAE
Neosartorya fischeri IRMRRFESLSLHEVCKGIKITSIPWL-----EHQESLPGRKLSLSDLHKRTELLHEFIYYIFDSILVPLIRSNFYVTESQIYRN-QLFYFRHDVWRHLTLQPLATFKATLFEEMKRADAE
Encephalitozoon cuniculi
IHRNRYETVSKNELVKYFRISRFKFFR-----------TLRCTRSEFVFRFRVSSRFLVYITEKLIIPIISKYFYCTETSFSKF-KVHYFPRSIWRHFSSIHIDRFLEKFEPAKG-----
730
740
750
760
770
780
790
800
810
820
830
840
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
------Motif 1---------| |-----------Motif
2-------------------|
|---------
Schizosaccharomyces pombe RMDTQKTTLPPAVIRLLPKKNT--FRLITNLRKRFLIK---------QMGSNKKMLVSTNQTLRPVAS-------ILKHLINEESSGIPFNLEVYMKLLTFKKDLLKHRMFG---RKKYF
Saccharomyces cerevisiae
HNSYTLSNFNHSKMRIIPKKSNNEFRIIAIPCRG------------ADEEEFTIYKENHKNAIQPT----QKILEYLRNKRPTSFTKIYSPTQIADRIKEFKQRLLKKFNN--ALPELYF
Saccharomyces paradoxus HNSYTLSNFNHSKMRIIPKKNNNEFRIIAIPCGG------------ADEEEFMIYKENHKNAVAPT----QKVLEYLRNKRQTSFTKMYSPMQIADRIKEFKQSLLKKFNN--VLPELYF
Saccharomyces mikatae HNSYTLSNFNHSRMRVIPKKSNNEFRIIAIPCGG------------ADDEEYMIYKENHKNAVAPT----QKILEYLRNKRLTSFTKIYSPTQIADRIKEFKLSLLKKFNN--VLPELYF
Saccharomyces kudriavzevii HNSYTLSNFNHCRMRIIPKKSNNEFRIIAIPCEG------------ADEEEFMVYKENHKRVIAPT----QKILEYLRNKRPTDFVKIYSPTQIADRIKEFKQGLLKKFNN--VLPELYF
Saccharomyces bayanus HNSYTLSNFNHSRMRIIPKKSNNEFRIIAIPCRG------------ADEEEFAVYKENHKKAVTPT----QKILEYLRKKRPTSFTKIYSPTQISDHIEKFKQILLKKFDN--ILPALYF
Kluyveromyces lactis HRSFTESKFNHRNLRLIPKKSGLDFRIIAVPCKG------------VNIQEFNEFNDYVTNALKPT----KIILERLRMRRNTKFTKAFSPLEIPKIVLSYKQRLLLKHG---KIPNLYL
Candida albicans 1 R-----EQLPCGKIKLIPKRSS--FRVICVPIKRSLKLLNKKLELDTLEKEKREFERYRKEVLSPVGQILRLKLSKLRDTYESYRASVHSSSDVAEKISDYRDSLLTRFG---EIPKLFI
Candida albicans 2 R-----EQLPCGKIKLIPKRSS--FRVICVPIKRSLKLLNKKLELDTLEKEKREFERYRKEVLSPVGQILRLKLSKLRDTYESYRASVHSSSDVAEKISDYRDSLLTRFG---EIPKLFI
Ashbya gossypii QILESR-DLGSSQIRLLPKGNR--LRPIMNLRRRAMAR----------G-QSRKLGLSINTVLGPIHS---LLKLER----------------------AFKGTLGPD------PGKLYF
Aspergillus fumigatus RRLCGR-SLGYSSLRLLPKTTG--VRPILNLRRRNLQP-------GWAG-KAPFLGPSINSTMTPIFN---VLKYETARNPASLGSTLSFVGEIHTRLKSFKERLSQS-HSSDRRKRLYF
Aspergillus clavatus RRLSSR-SLGYSSLRLLPKTTG--VRPILNLRRRMLKP-------GWAG-KAPFLGPSINSTIAPVFN---VLNYEKRRNPDSLGAALSFVGEIHTRLKGFKERLTQK-HAGHPRPHLYF
Aspergillus nidulans KLLSGKKSIGYGSLRLLPKTTG--IRPILNLRRRTLVRS------IYAGKNRYHPAQSVNSAIAPVYS---MLNYERGRRNDLLGSSMFSVGDMHSRLKKFKESLMSR--GWDQRKRLYF
Neosartorya fischeri RRLGGR-SLGYSSLRLLPKTTG--VRPILNLRRRMLQP-------GWAG-KAPFLGPSINSTITPIFN---VLNYEKARNPASLGSTLSFVGEIHTRLKSFKERLSQS-HSSDRRQPLYF
Encephalitozoon cuniculi
-----STTHSYSELRCIPKMNG--ARIISNMSKAR------------------NGRPSINKSIYPEFC------VLRHETHGMLGNSILNHSGMYEKLSSYLSRSVRP---------LYI
850
860
870
880
890
900
910
920
930
940
950
960
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
--Motif A-----------------|
Schizosaccharomyces pombe VRIDIKSCYDRIKQDLMFRIVKKKLKDPEFVIRKYATIHATS-----DRATKNFVSEAFSYFDMVP---------FEKVVQLLSMKTSDTLFVDFVDYWTKSSSEIFKMLKEHLSGHIVK
Saccharomyces cerevisiae
MKFDVKSCYDSIPRMECMRILKDALKNENG---FFVRS----------QYFFNTNTGVLKLFN---------------VVNASRVPKPYELYIDNVRTVHLSNQDVINVVEMEIFKTALW
Saccharomyces paradoxus MKFDVKSCYDSIPRMECMRILKEALKSENG---FFVRS----------QSFFNTNTGVLKLVN---------------VVNASRVPKPYELYIDNVRTIHLSNQDVINVVEMEIFKTALW
Saccharomyces mikatae MKFDVESCYDSIPRIECMRIIKRALKSENG---FFVRS----------QYISNTNTGALKLVN---------------VVNASRTPKPYELYIDNVRTVHLSNQDVINVIEMEIFKTALW
Saccharomyces kudriavzevii MKFDVKSCYDSIPRTECMRIIKRALKKENG---FFVRS----------QSFFNTNTGILKLTN---------------VVNASRTPKPYELYIDNVRTVHLSNQDVIDVIEMETFKTALW
Saccharomyces bayanus MKFDVKSCYDSIPRTKSMQIIKRALENENG---FFVRS----------QSFFNTNTGVLRLVD---------------VVNASRTPKPHELYIDNVRTVHLSNQDVINVIEMEMFKTSLW
Kluyveromyces lactis LKFDIQSCYDSIPVQKVMKLVEARICEEQQ---FFIRS----------QDILTTASTRLKRLH---------------AVNGDIQFQENDLVIDSVKTTVLSKEDVLTVVKMELFKTSIF
Candida albicans 1 LKFDMKECYDRLSQPVLMKKLEELFENQDNKTSYYVRYYAQLDASHKLKKVKTTIDTQYHNLN---------------ILSSSRHLSNCKSLVDKTKTIALQKGNILEVCRSQIYDVVGS
Candida albicans 2 LKFDMKECYDRLSQPVLMKKLEELFENQDNKTSYYVRYYAQLDASHKLKKVKTTIDTQYHNLN---------------ILSSSRHLSNCKSLVDKTKTIALQKGNILEVCRSQIYDVVGS
Ashbya gossypii AKTDVKAAFDTIPQEAVVELMKSVPSQTNYTIMKHVEVKPGE---RAVIDDDKYSSKAIRRWHATALSENESSVFVERLEKDLAPKKKNTVFVDSAFRRTKNVGELMHLLRQHVEQNLVL
Aspergillus fumigatus VKLDIQSCFDTIPQKELIPLVESLFSEEGYQIGKHVEVRPPDEFSSMWPVQESQQSKALRKYVGRAAPVSKAQALTDAITDGGISHRRNTVFVDTPAHKEYSGDYLLDLLDEHIRYNLVK
Aspergillus clavatus VKVDIQSCFDTIPQENLVPLVESLVSEEGYYVAKHVEVRPSDEFSSMWPLHESQQSKALRKYVGRAAPVTNSQDLTEAIAEGGISHRRNTVFVDTVAHKEYSGDYLLDLLDEHIRYNLVR
Aspergillus nidulans VKLDIQSCFDTIPQAKIVRLVEKLVSEENYHWMKYVEMRLASEFDNMWPLRKPQQRRTWSKYLQRVGPVGRPENLADAIANGSVVGRRNTVLVDTIAQKEYNGEGLLDILNEHIRNNLVK
Neosartorya fischeri VKLDIQSCFDTIPQEELIPLIESLFSEEGYQIGKHVEVRPPDEFSSMWPVQESQQSKALRKYVGRAAPVSRAQDLTDAITDGGISHRRNTVFVDTPAHKEYSGDYLLDLLDEHIRYNLVK
Encephalitozoon cuniculi
LKVDLSGCFDNIPQDEVVSLVRRLLHKSRYHTKSFSILEEVG--GELRSRYVCKVTENAETIN---------------ELMMEPGAFVNKVVKENASQRILSREEVEGAMTNMIKRNYVK
970
980
990
1000
1010
1020
1030
1040
1050
1060
1070
1080
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
|------------Motif B'------------------| |----Motif
C----||------Motif D------------|
|-Motif
Schizosaccharomyces pombe IGNS-----QYLQKVGIPQGSILSSFLCHFYMEDLIDEYLSFTKKK--GSVLLRVVDDFLFITVNKKDAKKFLNLSLRG-FEKHNFSTSLEKTVINFENSNGIINNTFFNESKKRMPFFG
Saccharomyces cerevisiae
VEDK-----CYIREDGLFQGSSLSAPIVDLVYDDLLEFYSEFKASPSQDTLILKLADDFLIISTDRQQVINIKKLAMGG-FQKYNAKANRDKILAVSSQSDDDTVIQ----------FCA
Saccharomyces paradoxus VEDK-----CYLREDGLFQGSSLSAPIVDLMYDDLLEFYGEFKTCPNQDTLILRLADDFLIISTDQQQIINIKNLASGG-FQKYNAKANEDKILAVSSHSDDDTVIQ----------FCA
Saccharomyces mikatae VQDK-----CYLREEGLFQGSCLSAPIVDLVYDDLLEFYDEFKSSSNQDTLILKLADDFLIISTDQQQIINVKKLAMDG-FEKYNAKVNRDKILSVCSPSDSGTVIQ----------FCA
Saccharomyces kudriavzevii VQDK-----CYLREDGLFQGSSLSAPIVDLVYDDLLEFYDEFKASPNQDTLILRLADDFLVISTDQQQISRIKKLAMGG-FEKYNAKANEDKIITVCSQPNNDTVIQ----------FCA
Saccharomyces bayanus IQDK-----CYLREDGLFQGSSLSAQIVDLVYDDLLESYNEFKASPNQDTLILRIADDFLIISTDQQQIIDIKNLATCG-FEEYNAKANEDKIITVSSQSNNDTIIQ----------FCA
Kluyveromyces lactis YRGK-----CYLRKDGLFQGTPLSSVLVDILYDDLLEFYTEFTDENDSDPMIIRLVDDFLIVSTSKKYIERLNNLAQTG-FKSFNTYINPQKVLVTSSESETSTNVP----------FCA
Candida albicans 1 VKDARGNLHLYKRKRGVFQGFSLSSIFCDILYSAMVHDCFQFLWKSKQDFLFVRLVDDFLLVTPDSNIYDQVHNILSGKILESYGAFVNKDKTVVVN-QTTTKTSID----------FVG
Candida albicans 2 VKDARGNLHLYKRKRGVFQGFSLSSIFCDILYSAMVHDCFQFLWKSKQDFLFVRLVDDFLLVTPDSNIYDQVHNILSGKILESYGAFVNKDKTVVVN-QTTTKPSID----------FVG
Ashbya gossypii VGKK-----YYRQKVGIPQGSVLSSFLCNYFYADLEAKHLGFLDSP--DCLLLRLIDDFLLVTLDQNKAAKFVNAMHGG-FPEYGVAVNPAKTMVNFDMLYAGEPVRNYNHEK-GFPYCG
Aspergillus fumigatus VGRK-----YFRQRNGIPQGSILSSVLCNLFYAQMERESLSFLDPS--EAVLLRLVDDFLLITINSNLAMRFLQVMLKG-QPKYGISINPAKSLVNFSAMVDGVQIPRLEGSH-LFPYCG
Aspergillus clavatus VGKK-----YFRQRNGIPQGSILSGVLCNLFYARMERECLGFLQSD--ESVLLRLIDDFLLVTTDSSLAMRFLQVMLKG-QPEYGITVNPAKSLVNFAAAVDGVQIPRLVDSA-LFPYCG
Aspergillus nidulans IGKK-----YFRQRKGIPQGSVLSSLLCSLLYAEMERDVLGFLQTD--DALLLRLLDDFLLVTLDSGLAMDFLRVMVRG-QPDYGISVNPAKSLVNFAAVVDGAQIPRLVDTP-LFPYCG
Neosartorya fischeri VGRK-----YFRQRNGIPQGSILSSVLCNLFYAQMERESLGFLDPS--EAVLLRLVDDFLLITTNSNLAMRFLQVMLKG-QPKYGISINPAKSLVNFSAAVDGVQIPRLEGSH-LFPYCG
Encephalitozoon cuniculi
HNGK-----LFVQKTGIAQGSVASTLLCSLYYKSIDDLYFDRVFKEG---ILTRYVDDFLVISPCIDEIMKFLEVSQSI--SHLGINFSKEKMESNFGLEEHLKAAS---------SISE
1090
1100
1110
1120
1130
1140
1150
1160
1170
1180
1190
1200
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
E-|
Schizosaccharomyces pombe FSVNMRSLDTLLACP-------KIDEALFNSTSVELTKHMGKSFFYKILRSSLASFAQVFIDITHNSKFNSCCNIYRLGYSMCMRAQAYLKRMKDIFIPQRMFITDLLNVIGRKIWKKLA
Saccharomyces cerevisiae
MHIFVKELEVWKHSS--------------TMNNFHIRSKSSKGIFRSLIALFNTRISYKTIDTNLNSTNTVLMQIDHVVKNISECYKSAFKDLSINVTQNMQFHSFLQRIIEMTVSGCPI
Saccharomyces paradoxus MHIFVKKLEVWKHSS--------------TMNNFNIRSSSSKRIFRSLIALFNTRISYKTVDSNLNSTTTVLMQIHHVVKNISEGYKSAFKDLSINDRESMQFSSFLLRIIEMTVDACPI
Saccharomyces mikatae MYISIKNLEVWKHSS--------------TMNIFNIHSSSSKKIFRSLIALFNTRLSYKTIDPDLNSITTVLMQIHHIVKNISESYKFAFRDLSINDTQNNHFSSFLLRIIEMTLDVYSI
Saccharomyces kudriavzevii MHIFIKELEVWKHSS--------------TMNIFNIRSNSSKKIFGRLISLFNTRISYKTADSNLNSITTVLMQIYHIVKNISESYKSAFKDLSMNDTQNIQFSSFLLRIIKMTLDVCLI
Saccharomyces bayanus MHIFIKELEIWKHSN--------------TMNVFNVRSNSSKIIFRRLTSLFNTRVSYKTINYNLNSTATVLMQIYYIVKNISEGYKFAFKDLPINNTQNVQFSLFLSNIIEMTLDICPA
Kluyveromyces lactis LQLNLRTLEVWKDRS--------------SYNVLGTVLGSSKQLYNKLLWIFEMRLSYNMLDERLNSWETAARFLREVALNVIQSFIVLFNRKKSSRRSFKQFVFNIFQRIDLNLSSANM
Candida albicans 1 LEVNTTDLSIKRNSG--------------SISLVTTNFRTFKTLVKYLKTFYQLNLEGFLLDCSFGVLENVLENMGSLLRLVLREFKTKFTSIVKYDT----FHCYKFIKFLYDISNYTI
Candida albicans 2 LEVNTTDLSIKRNSG--------------SISLVTTNFRTFKTLVKYLKTFYQLNLEGFLLDCSFGVLENVLENMGSLLRLVLREFKTKFTSIVKYDT----FHCYKFIKFLYDISNYTI
Ashbya gossypii TAINCQTLDITKDRERDAN-----------------------------IDAFKIQSHLMFYDTSHNSTRTVLSSLQGAFVETASKMLAYLRCLGKNQHPSSEMILRTIAKVIDVAYLLLT
Aspergillus fumigatus SLIDTRTLEIYRDQDRILEGTDSAAGTISSSLTVEFTRAPGRSLHRKVLSSFKLQMHPMYLDTKHNSLTVVLWNLYANLVTSAMKMYRYMKALPHRAHPTPEVVIRVIRDLIELAHRMMR
Aspergillus clavatus SLINTATLEIYRDPERVVEGGDSAAATLASSLTVESARAPGRSLHRKMLSAFKLQMHPMYLDTQHNSLSAVLSNLYASFVTAAMKMYQYMKALPRRAHPTAEVMARVIRDLMYLAHRMVG
Aspergillus nidulans SLIDTRTLEIFRDQDRMLEGADSASVALSDSLSIDSTRTPGRSFYRKVLASIKQSMHPMYLDSTHNSLPAVLLNVYKSFVTAAMKMYRYTRSLPGRARPRPEVVVRTIHDATQLGYRLIR
Neosartorya fischeri SLIDTRTLEIYRDQDRILEGTDSAAATISSSLTVESTRAPGRSLHRKVLSSFKLQMHPMYLDTKHNSLNVVLWNLYANLVTSAMKMYRYMKALPHRAHPTPDVVIRVIQDLIELAHRMMR
Encephalitozoon cuniculi
LSKHKHSMKITNAPVG------------------WCGTKIYSDGFSIKSQTADPYLPFSIAHSSTKPGRALESRMRKLLQNRMSRIYIDPNNKKAYENIYDTFLFYGKKLGLLLGRMDFI
1210
1220
1230
1240
1250
1260
1270
1280
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|..
structures
Schizosaccharomyces pombe EILG-----------YTSRRFLSSAEVKWLFCLGMRDGLKP-SFKYHPCFEQLIYQFQSLTDLIKPLRPVLRQVLFLHRRIAD----
Saccharomyces cerevisiae
TKCD----------------PLIEYEVRFTILNGFLESLSSNTSKFKDNIILLRKEIQHLQAYIYIYI-------------------
Saccharomyces paradoxus TKCD----------------PLIEYEVRFAILNGFLESLSSNASKFKNNIILLRKEIQHLQPHIYT---------------------
Saccharomyces mikatae TRLD----------------PLIGYEVRFTILNGFLKSFSSNTSKFKNNILFLEKEIQHLQMYVVS---------------------
Saccharomyces kudriavzevii TRCD----------------PLIEYEVRYTILNGFLESLCSNASRFKKNIIFLREEIQHLQAYI-----------------------
Saccharomyces bayanus MKYD----------------LFTEREVRCTILSGFLENLSSNTSKFKDNINYLRGEIQSLQVYI-----------------------
Kluyveromyces lactis FSME----------------KSVASKE--LVLSVFLEQLKLRKNAFADILTNVSDLKLLIEF-------------------------
Candida albicans 1 VKYV----------------ETNSDWEGAPELLNCIKQIIVKEFSSFESYSEIVEWVQTLNI-------------------------
Candida albicans 2 VKYV----------------ETNSDWDGAPELLNCIKQIIVKEFSSFESYSEIVEWVQTLNI-------------------------
Ashbya gossypii SKS-------RIMKYPQYTCDIRKSQVALTACLAFEKVLAAKQSNYQPVVKWLR---KEADRLASGRKHKSIHTPRSGQASG-----
Aspergillus fumigatus ARR---ELK-SRDSCSPYMCLVQQQQVRYLAAAAFRFVLGRKQTKYAKVLRWLEQVWKAARPQSDGKAARLAEVVRNGNSVFASWRF
Aspergillus clavatus ARR---ELHTARGPGSPSLCLVQGAQVQYLATAAFRFVLGRKQTKYAGVLRWLEQVWKAARPPSSAKAMRLAQVVRQGNASFAGWRF
Aspergillus nidulans GRHG--------------LCRVTHPQLQYLGGAAFQFVLGRKQTQYAGVLRWLDGTLAEAR-QSVGNSVLLAQAVQKGNRTYREWRF
Neosartorya fischeri ARR---ELK-SRDSCSPYMCLVQQQQVQYLAAAAFRFVLGRKQTKYAKVLRWLEQVWKAARPQSDGKAARLAQVVRNGNSVFASWRF
Encephalitozoon cuniculi
NRSFAR--------------RTLEHSKRFAFRICRKHGISITRKKIEDIASRAFDQSGVSRIVLTP---------------------